FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_6.3520000005fb41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_6.3520000005fb41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGATCAGACGTACTCTGCGTTGATACCAC421.9435446552521982No Hit
GTGCTACGATCAGACGTACTCTGCGTTGATACC281.2956964368347987No Hit
CTGCTACGATCAGACGTACTCTGCGTTGATACC160.7403979639055993No Hit
GATCAGACGTACTCAAAAAAAAAAAAAAAAAAA130.6015733456732995No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA120.5552984729291994No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.5090236001850995No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA80.37019898195279966No Hit
CTACGATCAGACGTACTCTGCGTTGATACCACT70.3239241092086997No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA70.3239241092086997No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA60.2776492364645997No Hit
GCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.2776492364645997No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT50.23137436372049977No Hit
CGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA50.23137436372049977No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA50.23137436372049977No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA50.23137436372049977No Hit
GTCTGATCGTAGCAAAAAAAAAAAAAAAAAAAA50.23137436372049977No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA40.18509949097639983No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA40.18509949097639983No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA40.18509949097639983No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18509949097639983No Hit
GTGCTACAAAAAAAAAAAAAAAAAAAAAAAAAA40.18509949097639983No Hit
ATGCTACGATCAGACGTACTCTGCGTTGATACC40.18509949097639983No Hit
GATCGTAGCAAAAAAAAAAAAAAAAAAAAAAAA40.18509949097639983No Hit
TGCTACGATCAGACGTACTCTGCGTTGATACCA40.18509949097639983No Hit
GTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.18509949097639983No Hit
GTGAATGGGGGCCTGCAAAAAAAAAAAAAAAAA30.13882461823229986No Hit
ACCCCCGCCTGCTACGATCAGACGTACTCTGCG30.13882461823229986No Hit
GATCAGACGTACAAAAAAAAAAAAAAAAAAAAA30.13882461823229986No Hit
GGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA30.13882461823229986No Hit
GTGAATGGGGTGCTACGAAAAAAAAAAAAAAAA30.13882461823229986No Hit
CTCCTGCTACGATCAGACGTACTCTGCGTTGAT30.13882461823229986No Hit
GACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA30.13882461823229986No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA30.13882461823229986No Hit
GTGTTGCTACGATCAGACGTACTCTGCGTTGAT30.13882461823229986No Hit
GATCAGACGAAAAAAAAAAAAAAAAAAAAAAAA30.13882461823229986No Hit
ACGCAGAGTGAATGGGGTCTGATCGTAGCAAAA30.13882461823229986No Hit
GACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA30.13882461823229986No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)