FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_56.3520000006018c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_56.3520000006018c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45796
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA13853.0242815966459955No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA6521.423705127085335No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC5101.113634378548345No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA4180.9127434710455061No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA3370.7358721285701808No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA3370.7358721285701808No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA3160.6900165953358373No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA2850.6223250938946633No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC2580.5633679797362215No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA2400.524063236963927No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA2270.4956764782950476No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.44982094506070397No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA2050.447637348240021No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA1870.40833260546772643No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA1580.34500829766791863No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC1510.3297231199231374No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA1380.301336361254258No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1350.2947855707922089No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC1250.27294960258537865No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA1210.2642152153026465No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA1120.24456284391649927No Hit
GTATCATCGCAGAGTGAATGGGCACTGTACGTA1060.23146126299240108No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA990.2161760852476199No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACA970.2118088916062538No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC930.2030745043235217No Hit
TATCTACGCAGAGTGAATGGGCACTGTACGTAC920.2008909075028387No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA900.19652371386147263No Hit
CATGAGCACTGTACGTACTCTTCGTTGATACCA880.19215652022010657No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA850.18560572975805747No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT830.1812385361166914No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA820.17905493929600838No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA800.17468774565464232No Hit
GCACTGTACGTACTAAAAAAAAAAAAAAAAAAA800.17468774565464232No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA750.1637697615512272No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA750.1637697615512272No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA740.16158616473054416No Hit
GTACGTACCTAAAAAAAAAAAAAAAAAAAAAAA720.1572189710891781No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT710.15503537426849506No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC700.15285177744781203No Hit
GTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA680.14848458380644597No Hit
GTACGTACCTGCAAAAAAAAAAAAAAAAAAAAA680.14848458380644597No Hit
CACATGAGCACTGTACGTACTCTGCGTTGATAC660.1441173901650799No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA660.1441173901650799No Hit
CAACGCAGAGTGAATGGGCACTGTACGTACAAA650.1419337933443969No Hit
GCATGAGCACTGTACGTACTCTGCGTTGATACC650.1419337933443969No Hit
GTACGTACCTGAAAAAAAAAAAAAAAAAAAAAA640.13975019652371387No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA620.1353830028823478No Hit
GAGCACTGTACGTACTCTGCGTTGATACCACTG600.13101580924098175No Hit
GCACTGTACGTACCTAAAAAAAAAAAAAAAAAA590.12883221242029871No Hit
GTACAGTGCTCAAAAAAAAAAAAAAAAAAAAAA590.12883221242029871No Hit
GAGTGAATGGGCACTGTACGTACTCAAAAAAAA580.12664861559961568No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA570.12446501877893265No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA560.12228142195824963No Hit
GCACTGTACAAAAAAAAAAAAAAAAAAAAAAAA540.11791422831688358No Hit
CACTGTACGTACAAAAAAAAAAAAAAAAAAAAA530.11573063149620054No Hit
GTACGTACAGTGCTCAAAAAAAAAAAAAAAAAA500.10917984103415146No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA500.10917984103415146No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA500.10917984103415146No Hit
GTCATGAGCACTGTACGTACTCTGCGTTGATAC500.10917984103415146No Hit
GTGAATGGGCACTGTACAAAAAAAAAAAAAAAA500.10917984103415146No Hit
TCCATGAGCACTGTACGTACTCTGCGTTGATAC500.10917984103415146No Hit
AATGGGCACTGTACGTACAAAAAAAAAAAAAAA470.10262905057210236No Hit
GAGTGAATGGGCACTGTACGTAAAAAAAAAAAA460.10044545375141935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGCA200.00853568826.993437
CTGCCAT200.00853568826.993435
CAACTCA257.723568E-426.9934271
GTATCAT455.407128E-826.9934271
TATCATC455.407128E-826.9934272
TACCTGC401.8070208E-523.6192516
TCGCAGA603.4635377E-822.4945227
ATCATCG553.763671E-722.0855333
ATCGCAG553.763671E-722.0855336
TCATCGC553.763671E-722.0855334
CATCGCA553.763671E-722.0855335
ATCTACG504.1020485E-621.5947422
TCTACGC504.1020485E-621.5947423
TACGCAG504.1020485E-621.5947425
TATCTAC504.1020485E-621.5947421
CTACGCA504.1020485E-621.5947424
GATACCA2150.021.36704427
CTGAGTG454.4880046E-520.9948884
GCTGAGT454.4880046E-520.9948883
TGAGTGA454.4880046E-520.9948885
Produced by FastQC (version 0.11.2)