FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_53.3520000006012f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_53.3520000006012f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31572
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2270.7189915114658558No Hit
ACGTAGCTCACGTACTCTGCGTTGATACCACTG2040.6461421512732801No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC1150.3642468009628785No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA980.31040162169010516No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA980.31040162169010516No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT970.30723425820347144No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC910.28823007728366906No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA900.28506271379703535No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA890.28189535031040164No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA850.2692258963638667No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA650.20587862663119222No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA630.19954389965792474No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA630.19954389965792474No Hit
GTGAATGGGTAGACGTAGAAAAAAAAAAAAAAA580.18370708222475612No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA480.15203344735841884No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA480.15203344735841884No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT430.13619662992525022No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA430.13619662992525022No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT420.13302926643861648No Hit
CCTTAGACGTAGCTCACGTACTCTGCGTTGATA400.12669453946534903No Hit
CATAGACGTAGCTCACGTACTCTGCGTTGATAC370.11719244900544787No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAGCTC370.11719244900544787No Hit
CCCTTAGACGTAGCTCACGTACTCTGCGTTGAT360.11402508551881414No Hit
GCCTAGACGTAGCTCACGTACTCTGCGTTGATA360.11402508551881414No Hit
GCTAGACGTAGCTCACGTACTCTGCGTTGATAC360.11402508551881414No Hit
ACGTAGCTCAAAAAAAAAAAAAAAAAAAAAAAA340.10769035854554668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.10452299505891297No Hit
GAGCTACGTCTAAAAAAAAAAAAAAAAAAAAAA330.10452299505891297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC1850.020.4180411
ATCAACG2100.017.9873222
TCAACGC2250.016.7881683
ATGGGAG500.002193443516.188595
GTTGATA1557.2759576E-1214.84306827
CGTTGAT3350.014.5433427
TGGGGAG657.403312E-414.5282226
GAGTACG600.00737367713.4904921
CAACGCA2800.013.0086884
GCGTTGA3850.013.0061126
ATGGGGA1001.0861654E-412.1414425
TGCGTTG4350.011.51115725
AACGCAG3250.011.2074865
CCCTAGA1000.001495360710.7923932
CTGCGTT4850.010.32443924
TCTGCGT7100.08.76810927
ACGCAGA4800.08.1505051
CGCAGAG4850.08.066482
AATGGGG3109.0636604E-87.8331894
AATGGGC1550.00605025147.8331894
Produced by FastQC (version 0.11.2)