FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_48.35200000060070.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_48.35200000060070.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3001
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA722.3992002665778074No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA120.39986671109630123No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA110.3665444851716095No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA100.33322225924691773No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA100.33322225924691773No Hit
GAGTGAATGGGGTTCCACAAAAAAAAAAAAAAA90.2999000333222259No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA80.26657780739753417No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA80.26657780739753417No Hit
GTTCCACGCTTAACGTACTCTTCGTTGATACCA80.26657780739753417No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA80.26657780739753417No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC70.23325558147284237No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA70.23325558147284237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.19993335554815062No Hit
CTGTTCCACGCTTAACGTACTCTGCGTTGATAC50.16661112962345886No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT50.16661112962345886No Hit
GAGTGAATGGGTTCCACAAAAAAAAAAAAAAAA50.16661112962345886No Hit
GTATCAACGCAGAGTGAATGGGTTCCACAAAAA50.16661112962345886No Hit
GTGAATGGGGTTCCACGCAAAAAAAAAAAAAAA50.16661112962345886No Hit
TATCAACGCAGAGTGAATGGGTTCCACGAAAAA50.16661112962345886No Hit
GAGTGAATGGGTTCCACGCAAAAAAAAAAAAAA50.16661112962345886No Hit
AACGCAGAGTGAATGGGGTTCCACAAAAAAAAA40.13328890369876709No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA40.13328890369876709No Hit
ACGCAGAGTACGTTAAAAAAAAAAAAAAAAAAA40.13328890369876709No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA40.13328890369876709No Hit
ACGCAGAGTGAATGGGGTTCCACAAAAAAAAAA40.13328890369876709No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA40.13328890369876709No Hit
GTTCCACAAAAAAAAAAAAAAAAAAAAAAAAAA40.13328890369876709No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCAAAA40.13328890369876709No Hit
ATCAACGCAGAGTGAATGGGTTCCACAAAAAAA40.13328890369876709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT708.742206E-413.527
CTCTGCG850.00379235711.11764726
ACTCTGC900.005821938610.525
TACTCTG900.005821938610.524
ACGCTTA1100.0020363869.81818214
AACGTAC1100.0020363869.81818220
CACGCTT1100.0020363869.81818213
TAACGTA1100.0020363869.81818219
CCACGCT1100.0020363869.81818212
CGCTTAA1100.0020363869.81818215
ACGTACT1100.0020363869.81818221
TTCCACG1100.0020363869.81818210
CTTAACG1100.0020363869.81818217
TTAACGT1100.0020363869.81818218
GCTTAAC1100.0020363869.81818216
GTTCCAC1150.00295277459.3913049
TCCACGC1150.00295277459.39130411
Produced by FastQC (version 0.11.2)