FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_45.35200000060013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_45.35200000060013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7023
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGAGATACGTACTCTGCGTTGATACCACTG200.2847785846504343No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.21358393848782573No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA140.19934500925530402No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA140.19934500925530402No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA130.18510608002278228No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA100.14238929232521716No Hit
GTGAATGGGGTAGCGTCAAAAAAAAAAAAAAAA100.14238929232521716No Hit
CCCTAGCGTCGAGATACGTACTCTGCGTTGATA90.12815036309269542No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA90.12815036309269542No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA80.11391143386017373No Hit
GTGAATGGGTAGCGTCGAAAAAAAAAAAAAAAA80.11391143386017373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT1201.5141086E-713.527
CTCTGCG1451.0574149E-712.10344726
ACTCTGC1552.5271402E-711.3225825
TACTCTG1552.5271402E-711.3225824
GTACTCT1655.697093E-710.63636323
GCGTCGA1801.4081525E-710.511
AGCGTCG1801.4081525E-710.510
TAGCGTC1801.4081525E-710.59
CGTACTC1708.3869963E-710.32352922
ACGTACT1852.058805E-710.21621621
CGTCGAG1852.058805E-710.21621612
TCGAGAT1852.058805E-710.21621614
GTCGAGA1902.9772673E-79.94736913
TACGTAC1902.9772673E-79.94736920
CGAGATA1902.9772673E-79.94736915
GAGATAC1902.9772673E-79.94736916
AGATACG1902.9772673E-79.94736917
GATACGT1954.2609645E-79.69230718
ATACGTA1954.2609645E-79.69230719
Produced by FastQC (version 0.11.2)