FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_42.3520000005ffc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_42.3520000005ffc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13445
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.19338043882484193No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA250.1859427296392711No Hit
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA220.16362960208255858No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA180.1338787653402752No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC150.11156563778356265No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG150.11156563778356265No Hit
ACTCTGCGTTGATACCAAAAAAAAAAAAAAAAA140.10412792859799182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT2054.9112714E-1111.88831827
GTGAATG900.00808868910.49126151
CCAGTCT1200.00635637348.992519
CTCTGCG2706.062328E-88.52485426
TGGAAAC2851.6083504E-88.51921916
TCTGGAA2952.9003786E-88.230432514
CTGGAAA2952.9003786E-88.230432515
GGAAACG3159.618816E-98.1360817
CTCTGGA3003.862806E-88.09325813
TCTCTGG3003.862806E-88.09325812
GAAACGT3201.2756573E-88.00895318
ACTCTGC3106.316441E-87.8616325
AAACGTA3302.2107997E-87.76625819
GTCTCTG3003.5319863E-77.643632411
AGTCTCT3003.5319863E-77.643632410
TACTCTG3251.4055149E-77.498785524
CAGTCTC3157.703293E-77.279659
AACGTAC3454.092417E-77.037616320
ACGTACT3454.092417E-77.037616321
GTACTCT3504.8798756E-76.963157723
Produced by FastQC (version 0.11.2)