FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_4.3520000005fb0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_4.3520000005fb0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3361
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA722.1422195775066943No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA290.8628384409401963No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA270.8033323415650104No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA260.7735792918774174No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA250.7438262421898245No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA160.47604879500148767No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA150.44629574531389465No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA150.44629574531389465No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA130.3867896459387087No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA110.32728354656352276No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.32728354656352276No Hit
GAGTGAATGGGTATCGACGAAAAAAAAAAAAAA100.2975304968759298No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA100.2975304968759298No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA100.2975304968759298No Hit
CAACGCAGAGTGAATGGGTATCGACGAAAAAAA90.2677774471883368No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA90.2677774471883368No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA80.23802439750074383No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA80.23802439750074383No Hit
ATCAACGCAGAGTGAATGGGTATCGACGTAAAA80.23802439750074383No Hit
GAGTGAATGGGGTATCGACGTCAAAAAAAAAAA80.23802439750074383No Hit
AACGCAGAGTGAATGGGTATCGACGTCAAAAAA80.23802439750074383No Hit
GTATCGACGTCTACGTACTCTTCGTTGATACCA80.23802439750074383No Hit
AACGCAGAGTGAATGGGTATCGACGTAAAAAAA80.23802439750074383No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA70.20827134781315082No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA70.20827134781315082No Hit
GTGAATGGGGTATCGACGAAAAAAAAAAAAAAA70.20827134781315082No Hit
GTATCGACGAAAAAAAAAAAAAAAAAAAAAAAA70.20827134781315082No Hit
CGTATCGACGTCTACGTACTCTGCGTTGATACC70.20827134781315082No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCT70.20827134781315082No Hit
GTGTATCGACGTCTACGTACTCTGCGTTGATAC70.20827134781315082No Hit
GGTATCGACGTCTACGTACTCTGCGTTGATACC60.17851829812555786No Hit
GAGTGAATGGGTATCGACGTCAAAAAAAAAAAA60.17851829812555786No Hit
GTGAATGGGGTATCGAAAAAAAAAAAAAAAAAA60.17851829812555786No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA60.17851829812555786No Hit
GTATCGACGTCTACAAAAAAAAAAAAAAAAAAA60.17851829812555786No Hit
GTGAATGGGTATCGAAAAAAAAAAAAAAAAAAA50.1487652484379649No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA50.1487652484379649No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA50.1487652484379649No Hit
GTGAATGGGGGTATCGAAAAAAAAAAAAAAAAA50.1487652484379649No Hit
GTATCGACGTCTACGTACAAAAAAAAAAAAAAA50.1487652484379649No Hit
TCTACGTACAAAAAAAAAAAAAAAAAAAAAAAA50.1487652484379649No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA50.1487652484379649No Hit
CCCGTATCGACGTCTACGTACTCTGCGTTGATA50.1487652484379649No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTAA50.1487652484379649No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCA50.1487652484379649No Hit
GTGAATGGGGGTATCGACGTCAAAAAAAAAAAA50.1487652484379649No Hit
ATCAACGCAGAGTGAATGGGTATCGACGTCTAC50.1487652484379649No Hit
GAGTGAATGGGTATCGACAAAAAAAAAAAAAAA50.1487652484379649No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGAAA50.1487652484379649No Hit
ATCAACGCAGAGTGAATGGGTATCGACGTCAAA40.11901219875037192No Hit
GTATCGACGTCTACGTACTCAAAAAAAAAAAAA40.11901219875037192No Hit
GGTATCGACAAAAAAAAAAAAAAAAAAAAAAAA40.11901219875037192No Hit
ACGCAGAGTGAATGGGGTATCGACAAAAAAAAA40.11901219875037192No Hit
GTGAATGGGTATCGACGTCTACGTACAAAAAAA40.11901219875037192No Hit
GAGTGAATGGGTATCGACGTAAAAAAAAAAAAA40.11901219875037192No Hit
GCGTATCGACGTCTACGTACTCTGCGTTGATAC40.11901219875037192No Hit
GTGAATGGGAGTATCGACAAAAAAAAAAAAAAA40.11901219875037192No Hit
ACCCAGTATCGACGTCTACGTACTCTGCGTTGA40.11901219875037192No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA40.11901219875037192No Hit
GTGAATGGGGTATCGACGTAAAAAAAAAAAAAA40.11901219875037192No Hit
GTACGTAGACGTCGAAAAAAAAAAAAAAAAAAA40.11901219875037192No Hit
AACGCAGAGTGAATGGGTATCGACGAAAAAAAA40.11901219875037192No Hit
GTGAATGGGTATCGACGTCTACGAAAAAAAAAA40.11901219875037192No Hit
GTGAATGGGGGTATCGACGAAAAAAAAAAAAAA40.11901219875037192No Hit
ACGCAGAGTGAATGGGGTATCGAAAAAAAAAAA40.11901219875037192No Hit
GTGAATGGGTATCGACGTCTACGTACTCAAAAA40.11901219875037192No Hit
GGGTATCGACGTCAAAAAAAAAAAAAAAAAAAA40.11901219875037192No Hit
GTATCGACGTCTACGAAAAAAAAAAAAAAAAAA40.11901219875037192No Hit
ACGCAGAGTGAATGGGTATCGACGTCTACAAAA40.11901219875037192No Hit
TATCAACGCAGAGTGAATGGGTATCGACAAAAA40.11901219875037192No Hit
CAACGCAGAGTGAATGGGAAGCAGTGGTATCAA40.11901219875037192No Hit
ACGCAGAGTGAATGGGGTATCGACGTCAAAAAA40.11901219875037192No Hit
GTGAATGGGAGTATCGACGAAAAAAAAAAAAAA40.11901219875037192No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGTAA40.11901219875037192No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC40.11901219875037192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA350.00448395219.28571527
TGCGTTG400.00970672616.87526
TCAACGC400.00970672616.8753
CTGCGTT400.00970672616.87525
GTGAATG1150.00312713259.3913041
Produced by FastQC (version 0.11.2)