FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_39.3520000005ff66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_39.3520000005ff66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGAGCTGACGAAAAAAAAAAAAAAAA190.5755831566192063No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT160.48470160557406844No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA150.45440775522568916No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC130.3938200545289306No Hit
AGCTGACGAAGTACGTACTCTGCGTTGATACCA120.36352620418055137No Hit
ACGCAGAGTGAATGGGAGCTGACGAAAAAAAAA110.3332323538321721No Hit
GTGAATGGGGCTGACGAAAAAAAAAAAAAAAAA110.3332323538321721No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA100.3029385034837928No Hit
ACGCAGAGTGAATGGGGCTGACGAAAAAAAAAA90.27264465313541353No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA90.27264465313541353No Hit
GAGCTGACGAAAAAAAAAAAAAAAAAAAAAAAA80.24235080278703422No Hit
GTACGTACTCTGCGTAAAAAAAAAAAAAAAAAA80.24235080278703422No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA70.21205695243865494No Hit
AACGCAGAGTGAATGGGAGCTGACGAAAAAAAA70.21205695243865494No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA70.21205695243865494No Hit
GAGCTGACGAAGTACAAAAAAAAAAAAAAAAAA70.21205695243865494No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.21205695243865494No Hit
GAGTGAATGGGAGCTGACGAAAAAAAAAAAAAA70.21205695243865494No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA60.18176310209027569No Hit
GTGAATGGGCTGACGAAAAAAAAAAAAAAAAAA60.18176310209027569No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT60.18176310209027569No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA60.18176310209027569No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA60.18176310209027569No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA60.18176310209027569No Hit
GAGTGAATGGGGCTGACGAAAAAAAAAAAAAAA60.18176310209027569No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG50.1514692517418964No Hit
GACGAAGTACGTACTCAAAAAAAAAAAAAAAAA50.1514692517418964No Hit
GTGTAGATCTCGGTGGTCGCCGTATCATTAAAA50.1514692517418964Illumina Single End PCR Primer 1 (100% over 29bp)
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC50.1514692517418964No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAAA50.1514692517418964No Hit
CAGCTGACGAAGTACGTACTCTGCGTTGATACC50.1514692517418964No Hit
AACGCAGAGTGAATGGGCTGACGAAAAAAAAAA50.1514692517418964No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT40.12117540139351711No Hit
ACGCAGAGTGAATGGGCTGACGAAAAAAAAAAA40.12117540139351711No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG40.12117540139351711No Hit
TAGTAGCGGCGAGCGAGAGCGGATTTGGGATTT40.12117540139351711No Hit
GGGCTGACGAAAAAAAAAAAAAAAAAAAAAAAA40.12117540139351711No Hit
GTATCAACGCAGAGTGAATGGGAGCTGACGAAG40.12117540139351711No Hit
GAGCTGACAAAAAAAAAAAAAAAAAAAAAAAAA40.12117540139351711No Hit
GAGCTGACGAAGTACGTACTCAAAAAAAAAAAA40.12117540139351711No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAGT40.12117540139351711No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA40.12117540139351711No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA40.12117540139351711No Hit
TATCAACGCAGAGTGAATGGGGCTGACGAAAAA40.12117540139351711No Hit
GAGTGAATGGGAGCTGACGAAGTACGTACAAAA40.12117540139351711No Hit
GTGAATGGGGCTGACAAAAAAAAAAAAAAAAAA40.12117540139351711No Hit
GAGTGAATGGGCTGACGAAAAAAAAAAAAAAAA40.12117540139351711No Hit
GTGAATGGGGTGGTATCAACGCAAAAAAAAAAA40.12117540139351711No Hit
GAGCTGACGAAGTACGTACTCTGCGTTGATACC40.12117540139351711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG200.0074447327.027
TCTGCGT758.348452E-514.427
CTCTGCG1006.1812374E-512.15000126
ACTCTGC1203.4117067E-410.12525
TACTCTG1350.00101631369.024
GTACTCT1400.00142060848.67857223
CGTACTC1400.00142060848.67857222
ACGTACT1500.00267365928.09999921
TACGTAC1500.00267365928.09999920
AAGTACG1550.0036058327.838709417
GTACGTA1550.0036058327.838709419
AGTACGT1600.0048126127.5937518
Produced by FastQC (version 0.11.2)