FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_36.3520000005ff09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_36.3520000005ff09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16625
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTTCTGACGTACTCTGCGTTGATACCAC1580.950375939849624No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.2947368421052632No Hit
CCACTGTCGTTCTGACGTACTCTGCGTTGATAC350.21052631578947367No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA190.1142857142857143No Hit
CTACTGTCGTTCTGACGTACTCTGCGTTGATAC190.1142857142857143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT3900.016.26923227
TATCAAC500.002124347516.21
ATCAACG500.002124347516.22
CTCTGCG4900.012.94897926
ACTCTGC4950.012.81818225
GACGTAC5200.012.46153920
TGACGTA5200.012.46153919
GTACTCT5100.012.44117623
CGTACTC5100.012.44117622
CTGACGT5250.012.34285718
TCTGACG5250.012.34285717
ACGTACT5150.012.32038921
TACTCTG5150.012.32038924
CACTGTC2450.012.1224498
TTCTGAC5400.012.016
TCGTTCT5400.012.013
GTCGTTC5450.011.88990712
GTTCTGA5450.011.88990715
TGTCGTT5450.011.88990711
CGTTCTG5450.011.88990714
Produced by FastQC (version 0.11.2)