FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_34.3520000005feb7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_34.3520000005feb7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25209
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA2811.1146812646277122No Hit
CATCGAGACGAGTACGTACTCTGCGTTGATACC570.22610972271807686No Hit
CGAGACGAGTACGTACTCTGCGTTGATACCACT370.14677297790471655No Hit
GTATTGTAAGTGGCAGAGTGGCCTTGCTGCCAC360.14280614066404856No Hit
GATCGAGACGAGTACGTACTCTGCGTTGATACC350.13883930342338055No Hit
CCATCGAGACGAGTACGTACTCTGCGTTGATAC290.11503827997937244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.11107144273870442No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC270.10710460549803642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT300.002204185122.5000024
ACATCGA350.00538715119.2857155
TCTGCGT2600.011.94230827
AATCGAG900.00838398110.5000018
CTCTGCG3303.6379788E-129.40909126
CTGCGTT1453.0882892E-49.31034626
TGCGTTG1350.0016974869.027
ACTCTGC3704.0017767E-118.391891525
ACGTACT4504.0017767E-117.521
TACTCTG4154.620233E-107.481927424
GAGTACG4555.0931703E-117.417582517
GTACTCT4205.9480953E-107.39285723
AGTACGT4606.548362E-117.336956518
CGTACTC4503.237801E-107.200000322
TACGTAC4751.364242E-107.10526320
CGAGTAC4751.364242E-107.10526316
GTACGTA4801.72804E-107.031250519
ACGAGTA4708.367351E-106.893616715
AGACGAG4604.0236046E-96.7513
GACGAGT4655.0404196E-96.67741914
Produced by FastQC (version 0.11.2)