FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_32.3520000005fe81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_32.3520000005fe81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5702
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA821.4380918975797967No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAC681.1925640126271484No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA560.9821115398105928No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA550.9645738337425466No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA460.8067344791301297No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA420.7365836548579445No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA390.6839705366538057No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA360.6313574184496668No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC280.4910557699052964No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.45598035776920376No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA250.4384426517011574No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA240.4209049456331112No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC240.4209049456331112No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA230.4033672395650649No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA220.3858295334970186No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT210.36829182742897226No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA210.36829182742897226No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA200.350754121360926No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA170.2981410031567871No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA170.2981410031567871No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.28060329708874077No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA160.28060329708874077No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG160.28060329708874077No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA160.28060329708874077No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA150.2630655910206945No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA150.2630655910206945No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA150.2630655910206945No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA140.2455278849526482No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA140.2455278849526482No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA130.22799017888460188No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA130.22799017888460188No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA130.22799017888460188No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.2104524728165556No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA120.2104524728165556No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.2104524728165556No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA120.2104524728165556No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA110.1929147667485093No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC100.175377060680463No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA100.175377060680463No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA100.175377060680463No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA100.175377060680463No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA100.175377060680463No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA100.175377060680463No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA90.1578393546124167No Hit
GAGTGAATGGGGCTAACTATCCACGTACAAAAA90.1578393546124167No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA80.14030164854437038No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA80.14030164854437038No Hit
GAGTGAATGGGCTAACTATAAAAAAAAAAAAAA80.14030164854437038No Hit
ATCAACGCAGAGTGAATGGGGCTAACTATCCAC80.14030164854437038No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA80.14030164854437038No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA80.14030164854437038No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA80.14030164854437038No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA70.1227639424763241No Hit
GAGTGAATGGGCTAACTAAAAAAAAAAAAAAAA70.1227639424763241No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC70.1227639424763241No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC70.1227639424763241No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC70.1227639424763241No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA70.1227639424763241No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA70.1227639424763241No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCAAA60.1052262364082778No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA60.1052262364082778No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA60.1052262364082778No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
CGGCTAACTATCCACGTACTCTGCGTTGATACC60.1052262364082778No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACAAA60.1052262364082778No Hit
CTAACTATCCACGTACTCTGCGTTGATACCACT60.1052262364082778No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA60.1052262364082778No Hit
AACTATCCACAAAAAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
TATCAACGCAGAGTGAATGGGCTAACTATAAAA60.1052262364082778No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
ACGCAGAGTGAATGGGGCTAACTAAAAAAAAAA60.1052262364082778No Hit
AACTATCCACGTACTCAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
GTGCAGATCTTGGTGGTAGTAGCAAAAAAAAAA60.1052262364082778No Hit
CTATCCACGTACCAAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
AACTATCCACGTACAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC557.3268657E-619.5980851
TCAACGC500.001925680816.168423
ATCAACG500.001925680816.168422
CAACGCA500.001925680816.168424
AACGCAG550.003640211714.6985655
TCTGCGT1252.0260268E-713.04920427
ACGCAGA850.004710051711.0959751
CTCTGCG1552.7672868E-610.52355226
ACTCTGC1655.8703317E-69.8857625
CAGAGTG1150.00386992969.3729979
AGAGTGA1150.00386992969.37299710
GTACTCT1801.6609076E-59.06194723
TACTCTG1852.300607E-58.8170324
GTGAATG1903.522176E-58.5096951
TGAATGG1954.7878115E-58.2914982
GAATGGG2101.1443201E-47.69924833
ATCCACG2406.125466E-57.298245417
CGTACTC2252.298178E-47.249557522
TAACTAT2805.7930356E-67.21804512
AACTATC2805.7930356E-67.21804513
Produced by FastQC (version 0.11.2)