FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_31.3520000005fe67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_31.3520000005fe67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22448
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGATGTCTGACGTACTCTGCGTTGATACC280.12473271560940842No Hit
CCCTTGCGATGTCTGACGTACTCTGCGTTGATA250.11136849607982895No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11136849607982895No Hit
TGCGATGTCTGACGTACTCTGCGTTGATACCAC230.10245901639344263No Hit
CTTGCGATGTCTGACGTACTCTGCGTTGATACC230.10245901639344263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC401.7574886E-523.6132811
ATGGGAC300.002202891722.488845
TGGGACA300.002202891722.488846
TCAACGC552.0384611E-417.1732963
ATCAACG552.0384611E-417.1732962
CAACGCA552.0384611E-417.1732964
AACGCAG657.27319E-414.5312495
AATGGGA700.001274143813.4933044
TGCGTTG1307.749273E-611.44295227
GTGAATG1452.6673419E-510.23629951
TGAATGG1555.5570723E-59.5758922
CTGCGTT1607.676684E-59.297399526
TCTGCGT2552.960769E-89.0156627
GAATGGG1701.5243105E-48.7309623
CTCTGCG3151.042863E-77.72770926
ACTCTGC3651.224591E-66.66911925
ACGTACT4152.1402047E-76.50279721
TGACGTA4202.6609814E-76.425382619
TACTCTG3802.3798675E-66.40586424
CTGACGT4253.2986281E-76.349789618
Produced by FastQC (version 0.11.2)