FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_3.3520000005fae2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_3.3520000005fae2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTTCCTCACGTACTCTGCGTTGATACCA1003.204101249599487No Hit
CTCTGTTCCTCACGTACTCTGCGTTGATACCAC240.768984299903877No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA90.28836911246395386No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT60.19224607497596924No Hit
ACTCTGTTCCTCACGTACTCTTCGTTGATACCA60.19224607497596924No Hit
CCCCCACTCTGTTCCTCACGTACTCTGCGTTGA60.19224607497596924No Hit
CTACTCTGTTCCTCACGTACTCTGCGTTGATAC60.19224607497596924No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA50.16020506247997437No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA50.16020506247997437No Hit
GAATGGGACTCTGTAAAAAAAAAAAAAAAAAAA50.16020506247997437No Hit
GCCCTACTCTGTTCCTCACGTACTCTGCGTTGA50.16020506247997437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.16020506247997437No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA40.12816404998397948No Hit
GTACTCTGTTCCTCACGTACTCTGCGTTGATAC40.12816404998397948No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA40.12816404998397948No Hit
CCGCCCACTCTGTTCCTCACGTACTCTGCGTTG40.12816404998397948No Hit
GCACTCTGTTCCTCACGTACTCTGCGTTGATAC40.12816404998397948No Hit
GTGAATGGGACTCTGTTCCAAAAAAAAAAAAAA40.12816404998397948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA300.001798006222.524
GATACCA300.001798006222.527
TGATACC300.001798006222.526
TTGATAC300.001798006222.525
CGTTGAT300.001798006222.523
GCGTTGA600.005550162413.527
Produced by FastQC (version 0.11.2)