FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_26.3520000005fdc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_26.3520000005fdc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9724
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACAGACAGTACGTACTCTGCGTTGATACCA2492.560674619498149No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT1461.5014397367338543No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA650.6684491978609626No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA550.5656108597285068No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.5450431921020157No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA480.49362402303578773No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA420.43192102015631423No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA390.4010695187165776No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA380.390785684903332No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA360.3702180172768408No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA340.34965034965034963No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA320.3290826820238585No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA290.29823118058412174No Hit
ACAGTACGTACTCTGCGTTGATACCACTGCTTC290.29823118058412174No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA280.2879473467708762No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA280.2879473467708762No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA250.25709584533113944No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA240.24681201151789386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.22624434389140274No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC210.21596051007815711No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA200.20567667626491154No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA190.195392842451666No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA190.195392842451666No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.195392842451666No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA190.195392842451666No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA180.1851090086384204No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA180.1851090086384204No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA170.17482517482517482No Hit
GTGAATGGGACACAGACAGTACAAAAAAAAAAA170.17482517482517482No Hit
GTGAATGGGACACAAAAAAAAAAAAAAAAAAAA170.17482517482517482No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.15425750719868367No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA150.15425750719868367No Hit
CAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.15425750719868367No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.15425750719868367No Hit
AGACAGTACAAAAAAAAAAAAAAAAAAAAAAAA140.1439736733854381No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1439736733854381No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA140.1439736733854381No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA140.1439736733854381No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA130.1336898395721925No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1336898395721925No Hit
CAACTCAGAGTGAATGGGACACAGACAGAAAAA130.1336898395721925No Hit
ATCAACGCAGAGTGAATGGGACACAGACAGTAC120.12340600575894693No Hit
GAGTAGGGGTCGCGAGAGAGCAGAGCGTACCGC120.12340600575894693No Hit
GTGAATGGGGACACAGACAGAAAAAAAAAAAAA120.12340600575894693No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGA110.11312217194570137No Hit
GACACAGACAGTACGTAAAAAAAAAAAAAAAAA110.11312217194570137No Hit
GGACACAGACAGTACGTACTCTGCGTTGATACC110.11312217194570137No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA110.11312217194570137No Hit
ACGCAGAGTGAATGGGACACAGACAGTAAAAAA110.11312217194570137No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA110.11312217194570137No Hit
GTTTATAGTCGCGACTCTTGTCATAGTCAAGAA110.11312217194570137No Hit
CAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAA100.10283833813245577No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAAA100.10283833813245577No Hit
ATCAACGCAGAGTGAATGGGACACAGACAAAAA100.10283833813245577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTG200.00804196527.10880925
CACTGCT200.00804196527.10880927
CCACTGC200.00804196527.10880926
TACCACT257.107572E-427.10880924
ATACCAC300.002058443822.59067323
TCTGCGT1902.073648E-911.41423527
CTCTGCG2056.855771E-910.57904726
ACTCTGC2202.0696461E-89.85774825
TGGGACA1257.5213995E-49.7088666
TACTCTG2304.134199E-89.4291524
GTACTCT2355.772017E-89.2285323
ATGGGAC1250.0085450498.6301035
AGTACGT2757.289054E-88.33589518
TACGTAC2757.289054E-88.33589520
AGACAGT2952.3819666E-88.22785214
GTACGTA2809.7637894E-88.1870419
CGTACTC2653.6750316E-78.18379122
ACGTACT2705.3090116E-77.99083621
GACAGTA3054.209687E-87.95808715
ACAGTAC2901.7223465E-77.90472816
Produced by FastQC (version 0.11.2)