FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_25.3520000005fdac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_25.3520000005fdac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5762
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGAACCAAGACGTACTCTGCGTTGATACCA220.3818118708781673No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA100.17355085039916696No Hit
AACCAAGACGTACTCAAAAAAAAAAAAAAAAAA100.17355085039916696No Hit
GTATTGTAAGTGGCAGAGTGGCCTTGCTGCCAC80.13884068031933355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.13884068031933355No Hit
ACGCAGAGTGAATGGGCAACGAACAAAAAAAAA80.13884068031933355No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA70.12148559527941688No Hit
GAGTGAATGGGCAACGAACCAAAAAAAAAAAAA70.12148559527941688No Hit
GTATCAACGCAGAGTGAATGGGCAACGAACCAA60.10413051023950018No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)