FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_24.3520000005fd82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_24.3520000005fd82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11404
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACTACACACGTACTCTGCGTTGATACCAC1461.28025254296738No Hit
CCTCCACACTACACACGTACTCTGCGTTGATAC1361.1925640126271484No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC690.6050508593475974No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC650.5699754472115047No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC580.5085934759733427No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC530.46474921080322695No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC520.45598035776920376No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC410.3595229743949492No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC390.34198526832690285No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC390.34198526832690285No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC310.2718344440547177No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC300.2630655910206945No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC230.20168361978253244No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC220.1929147667485093No Hit
ATCCACACTACACACGTACTCTGCGTTGATACC220.1929147667485093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.175377060680463No Hit
CCCCTCCACACTACACACGTACTCTGCGTTGAT200.175377060680463No Hit
TGTCCACACTACACACGTACTCTGCGTTGATAC160.14030164854437038No Hit
TTTCCACACTACACACGTACTCTGCGTTGATAC160.14030164854437038No Hit
AATCCACACTACACACGTACTCTGCGTTGATAC160.14030164854437038No Hit
TATCCACACTACACACGTACTCTGCGTTGATAC140.1227639424763241No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA140.1227639424763241No Hit
AGTCCACACTACACACGTACTCTGCGTTGATAC130.11399508944230094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA200.00826382627.026
ATACCAC200.00826382627.027
CATCCAC300.00213100922.57
GTTGATA952.1827873E-1119.89473726
TTGATAC1004.5474735E-1118.927
CGTTGAT1059.094947E-1118.025
GCGTTGA1206.1299943E-1015.7524
ATCCACA903.5928366E-513.58
TGCGTTG1501.4359102E-812.59999923
CTGCGTT1655.4418706E-811.45454622
TCTGCGT2800.011.08928627
CTCTGCG3150.09.85714326
CTCCACA1606.948423E-59.281258
ACTCTGC3403.6379788E-129.13235325
CGTACTC3907.2759576E-128.30769322
TACTCTG3753.092282E-118.2824
GTACTCT3804.0017767E-118.17105323
ACGTACT4001.2732926E-118.09999921
CACACGT4102.3646862E-117.902438618
CACGTAC4102.3646862E-117.902438620
Produced by FastQC (version 0.11.2)