FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_22.3520000005fd4f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_22.3520000005fd4f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15966
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC800.5010647626205688No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC700.43843166729299765No Hit
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA680.42590504822748343No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC530.33195540523612677No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA510.31942878617061254No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA480.3006388575723412No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA450.2818489289740699No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA330.20668921458098458No Hit
AGGTAGTTCTGACACGTACTCTGCGTTGATACC320.2004259050482275No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA310.19416259551547038No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.18789928598271327No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA300.18789928598271327No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA280.17537266691719905No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC270.16910935738444194No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA260.16284604785168483No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA260.16284604785168483No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA240.1503194287861706No Hit
TGGTAGTTCTGACACGTACTCTGCGTTGATACC240.1503194287861706No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT240.1503194287861706No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA220.1377928097206564No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA190.11900288112238508No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA180.11273957158962795No Hit
GTAGTTCTGACACGTACTCTGCGTTGATACCAC180.11273957158962795No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.11273957158962795No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA180.11273957158962795No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA170.10647626205687086No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA170.10647626205687086No Hit
GTGAATGGGTAGTTCTGACACGTAAAAAAAAAA160.10021295252411375No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAG160.10021295252411375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC454.2712956E-520.9853731
GTTGATA350.005226952519.33288425
TGATACC350.005226952519.33288427
TTGATAC350.005226952519.33288426
TCAACGC551.9950299E-417.169853
ATCAACG551.9950299E-417.169852
TGCGTTG1401.8189894E-1216.43295127
TGGGGTA500.002129644416.1887156
CAACGCA657.1213255E-414.5283354
TCTGCGT3950.012.67649827
GGGGTAG750.002098788712.5912237
AGGTAGT1358.505758E-711.991648
CTGCGTT2055.4569682E-1111.8826526
CTCTGCG4400.011.68760826
AACGCAG850.00535886911.1099025
ACTCTGC4800.010.7136425
GTACTCT5050.010.45124223
TACTCTG5050.010.45124224
CGTACTC5350.010.371098522
TGGGTAG1050.002141511310.2785496
Produced by FastQC (version 0.11.2)