FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_21.3520000005fd18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_21.3520000005fd18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4501
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.5332148411464119No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC200.4443457009553432No Hit
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA150.33325927571650743No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA120.26660742057320597No Hit
CACCAGTTCAAGGTACGTACTCTGCGTTGATAC100.2221728504776716No Hit
GCCCAGTTCAAGGTACGTACTCTGCGTTGATAC100.2221728504776716No Hit
GTCCAGTTCAAGGTACGTACTCTGCGTTGATAC100.2221728504776716No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA100.2221728504776716No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA90.19995556542990447No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA90.19995556542990447No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA80.1777382803821373No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT70.15552099533437014No Hit
CAGTTCAAGGTACGTACTCTGCGTTGATACCAC70.15552099533437014No Hit
TATCAACGCAGAGTGAATGGGCCAGTTCAAGGT70.15552099533437014No Hit
GCCCAGTTCAAGGAAAAAAAAAAAAAAAAAAAA70.15552099533437014No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT60.13330371028660298No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA60.13330371028660298No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA60.13330371028660298No Hit
ACCCAGTTCAAGGTACGTACTCTGCGTTGATAC60.13330371028660298No Hit
ACTCTGCGTTGATACCAAAAAAAAAAAAAAAAA50.1110864252388358No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA50.1110864252388358No Hit
ACGCAGAGTGAATGGGCCAGTTCAAGGTAAAAA50.1110864252388358No Hit
CTCCAGTTCAAGGTACGTACTCTGCGTTGATAC50.1110864252388358No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA50.1110864252388358No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1110864252388358No Hit
GTACGTACCTTGAAAAAAAAAAAAAAAAAAAAA50.1110864252388358No Hit
ATCCAGTTCAAGGTACGTACTCTGCGTTGATAC50.1110864252388358No Hit
TCCCAGTTCAAGGTACGTACTCTGCGTTGATAC50.1110864252388358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC351.6347147E-423.1428591
TCTGCGT1550.019.1612927
TCAACGC458.9437363E-418.0000023
ATCAACG458.9437363E-418.0000022
CAACGCA458.9437363E-418.0000024
CTCTGCG1850.016.05405426
GCCAGTT550.003432757514.7272738
ACTCTGC2200.013.525
CAGAGTG600.006126360513.4999999
GTACTCT2150.013.18604723
CGTACTC2150.013.18604722
TACTCTG2150.013.18604724
ACGTACT2200.012.88636421
TACGTAC2350.012.63829820
AGGTACG2350.012.63829817
GTACGTA2350.012.63829819
GGTACGT2250.012.618
CCCAGTT750.001707299112.5999998
AAGGTAC2400.012.37499916
TCAAGGT2500.011.87999914
Produced by FastQC (version 0.11.2)