FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_20.3520000005fcfd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_20.3520000005fcfd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6542
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGAGACTACGTACTCTGCGTTGATACCACT1011.5438703760317947No Hit
ACGTAGGAGACTACGTACTCTGCGTTGATACCA971.4827269948028126No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA360.5502904310608377No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA320.4891470498318557No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA240.36686028737389176No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC220.3362885967594008No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.32100275145215534No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA200.3057169061449098No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA190.2904310608376643No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA180.2751452155304189No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA170.25985937022317335No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA160.24457352491592785No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA150.22928767960868238No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA150.22928767960868238No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.22928767960868238No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA130.19871598899419135No Hit
CCACGTAGGAGACTACGTACTCTGCGTTGATAC130.19871598899419135No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA130.19871598899419135No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA120.18343014368694588No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.1681442983797004No Hit
GACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAA100.1528584530724549No Hit
ACGTAGGAGACTACGTACTCTTCGTTGATACCA100.1528584530724549No Hit
GTGAATGGGACGTAGGAAAAAAAAAAAAAAAAA100.1528584530724549No Hit
ATCAACGCAGAGTGAATGGGACGTAGGAGAAAA90.13757260776520944No Hit
CACGTAGGAGACTACGTACTCTGCGTTGATACC90.13757260776520944No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA90.13757260776520944No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA90.13757260776520944No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA90.13757260776520944No Hit
GTGAATGGGAACGTAGGAGACAAAAAAAAAAAA80.12228676245796392No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAC80.12228676245796392No Hit
GCACGTAGGAGACTACGTACTCTGCGTTGATAC80.12228676245796392No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA80.12228676245796392No Hit
GAACGTAGGAGACTACGTACTCTGCGTTGATAC80.12228676245796392No Hit
AACGCAGAGTGAATGGGACGTAGGAGAAAAAAA80.12228676245796392No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA70.10700091715071844No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA70.10700091715071844No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA70.10700091715071844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC557.702847E-619.5692311
ATCAACG601.6339478E-517.9384612
CAACGCA706.14811E-515.3758254
TCAACGC751.1089275E-414.350773
AACGCAG1000.001264250610.7630785
TCTGCGT1204.115666E-410.24804727
ACGCAGA1256.959762E-49.68676956
CGCAGAG1256.959762E-49.68676957
GCAGAGT1200.0057606568.9692318
CTCTGCG1450.00234280858.48114226
ACTCTGC1550.0042928767.93397225
GTACTCT1650.00754480257.45312523
TACTCTG1650.00754480257.45312524
CGTACTC1700.0098624997.233915322
Produced by FastQC (version 0.11.2)