FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_19.3520000005fce0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_19.3520000005fce0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9783
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA550.562199734232853No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.5519779208831647No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA470.48042522743534705No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC450.45998160073597055No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA400.40887253398752943No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA350.35776346723908825No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA330.33731984053971176No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA320.3270980271900235No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA290.2964325871409588No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA290.2964325871409588No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA260.2657671470918941No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA260.2657671470918941No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA260.2657671470918941No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA260.2657671470918941No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA260.2657671470918941No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA260.2657671470918941No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA250.2555453337422059No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA250.2555453337422059No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA240.24532352039251765No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.24532352039251765No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA240.24532352039251765No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA230.23510170704282937No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC230.23510170704282937No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA200.20443626699376471No Hit
AATGCGCTCTGAACGTACTCTGCGTTGATACCA200.20443626699376471No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA190.19421445364407647No Hit
GTGAATGGGAATGCGCTCAAAAAAAAAAAAAAA170.1737708269447No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACAAAAA170.1737708269447No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAAAAAA160.16354901359501176No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA150.1533272002453235No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA150.1533272002453235No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT140.1431053868956353No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA140.1431053868956353No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA130.13288357354594704No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA130.13288357354594704No Hit
GAGTGAATGGGCTCTGAACGTACTCTGCGTTGA120.12266176019625882No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGAAAA120.12266176019625882No Hit
GAATGCGCTCTGAACGTACTCTGCGTTGATACC120.12266176019625882No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT120.12266176019625882No Hit
GAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.12266176019625882No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA110.11243994684657058No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA110.11243994684657058No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA100.10221813349688236No Hit
AACGCAGAGTGAATGGGCTCTGAAAAAAAAAAA100.10221813349688236No Hit
GTGAATGGGCTCTGAACGTACTCTGCGTTGATA100.10221813349688236No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA100.10221813349688236No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA100.10221813349688236No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACAAAA100.10221813349688236No Hit
GAATGGGCTCTGAACAAAAAAAAAAAAAAAAAA100.10221813349688236No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA100.10221813349688236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG404.6125444E-420.2115382
TATCAAC509.208854E-518.8641031
TCAACGC600.00695642813.47435953
CAACGCA600.00695642813.47435954
TGCGTTG1359.810114E-611.03570127
CTGCGTT1452.1693879E-510.27461826
CAATGCG1205.2039337E-410.105778
TCTGCGT2304.1978637E-89.42178427
ACGCAGA1200.00615130238.9829066
CGCAGAG1200.00615130238.9829067
CAGAGTG1250.0086049778.62358959
AGAGTGA1250.0086049778.623589510
CTCTGCG3002.4579276E-67.223367726
ACTCTGC3258.168565E-66.667723725
GCGCTCT3452.8004724E-66.639539212
TACTCTG3401.5982076E-56.373559524
TGCGCTC3605.4716893E-66.362891711
CGCTCTG3605.4716893E-66.362891713
ATGCGCT3656.792181E-66.275728710
AATGCGC3708.402887E-66.1909229
Produced by FastQC (version 0.11.2)