FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_17.3520000005fcad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_17.3520000005fcad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13984
Sequences flagged as poor quality0
Sequence length33
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACGCACGTTACGTACTCTGCGTTGATACCA1591.1370137299771166No Hit
CCATACGCACGTTACGTACTCTGCGTTGATACC790.5649313501144165No Hit
ATACGCACGTTACGTACTCTGCGTTGATACCAC560.40045766590389015No Hit
CCCATACGCACGTTACGTACTCTGCGTTGATAC530.3790045766590389No Hit
GCATACGCACGTTACGTACTCTGCGTTGATACC400.2860411899313501No Hit
ACCATACGCACGTTACGTACTCTGCGTTGATAC270.19307780320366133No Hit
CTCATACGCACGTTACGTACTCTGCGTTGATAC250.17877574370709384No Hit
GCCATACGCACGTTACGTACTCTGCGTTGATAC240.17162471395881007No Hit
ACATACGCACGTTACGTACTCTGCGTTGATACC230.1644736842105263No Hit
GTCATACGCACGTTACGTACTCTGCGTTGATAC230.1644736842105263No Hit
ATCATACGCACGTTACGTACTCTGCGTTGATAC190.1358695652173913No Hit
CACATACGCACGTTACGTACTCTGCGTTGATAC170.1215675057208238No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.1072654462242563No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA140.10011441647597254No Hit
CGCATACGCACGTTACGTACTCTGCGTTGATAC140.10011441647597254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA306.545588E-527.0755427
TGATACC500.002062223216.24532326
TTGATAC550.003894575614.76847625
TCTGCGT1607.0410286E-59.30721727
CTCTGCG1802.5407673E-48.27308226
ACTCTGC1956.022125E-47.63669125
TACTCTG2050.00102836727.26416924
GTACTCT2200.00217689486.768884723
CGTACTC2300.0034776256.47458522
CGTTACG2400.005641956.182571417
CACGTTA2400.005641956.182571415
ACGTTAC2400.005641956.182571416
GCACGTT2400.005641956.182571414
GTTACGT2400.005641956.182571418
ACGTACT2450.00699411056.056396521
TACGTAC2450.00699411056.056396520
CGCACGT2450.00699411056.056396513
TTACGTA2450.00699411056.056396519
CATACGC2700.00279505955.99522079
Produced by FastQC (version 0.11.2)