FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_16.3520000005fc83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_16.3520000005fc83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4502
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATGGTGTTCACGTACTCTGCGTTGATACCA741.6437139049311416No Hit
GTGAATGGGCTATGGTGAAAAAAAAAAAAAAAA481.0661928031985786No Hit
CCGCTATGGTGTTCACGTACTCTGCGTTGATAC400.888494002665482No Hit
CTATGGTGTTCACGTACTCTGCGTTGATACCAC360.7996446023989338No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGAAAA270.5997334517992003No Hit
GTGAATGGGCTATGGTGTAAAAAAAAAAAAAAA260.5775211017325633No Hit
ACGCAGAGTGAATGGGCTATGGTGAAAAAAAAA230.5108840515326522No Hit
GTGTTCACGTACTCAAAAAAAAAAAAAAAAAAA180.3998223011994669No Hit
GCGCTATGGTGTTCACGTACTCTGCGTTGATAC170.37760995113282986No Hit
CTGCTATGGTGTTCACGTACTCTGCGTTGATAC170.37760995113282986No Hit
GTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.37760995113282986No Hit
GGGCTATGGTGAAAAAAAAAAAAAAAAAAAAAA160.3553976010661928No Hit
GTGAATGGGGCTATGGTGAAAAAAAAAAAAAAA160.3553976010661928No Hit
GTGCTATGGTGTTCACGTACTCTGCGTTGATAC150.33318525099955576No Hit
AACGCAGAGTGAATGGGCTATGGTGAAAAAAAA150.33318525099955576No Hit
GGGCTATGGTGTTCACGTACTCTGCGTTGATAC140.3109729009329187No Hit
GAGCTATGGTGTTCACGTACTCTGCGTTGATAC140.3109729009329187No Hit
CAGCTATGGTGTTCACGTACTCTGCGTTGATAC140.3109729009329187No Hit
ACGCAGAGTGAATGGGCTATGGTGTAAAAAAAA140.3109729009329187No Hit
GTGAATGGGCTATGGTAAAAAAAAAAAAAAAAA130.28876055086628166No Hit
ATGCTATGGTGTTCACGTACTCTGCGTTGATAC130.28876055086628166No Hit
GGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.28876055086628166No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTAAA130.28876055086628166No Hit
TTGCTATGGTGTTCACGTACTCTGCGTTGATAC130.28876055086628166No Hit
GCTATGGTGAAAAAAAAAAAAAAAAAAAAAAAA120.26654820079964464No Hit
GAATGGGCTATGGTGAAAAAAAAAAAAAAAAAA120.26654820079964464No Hit
GAGTGAATGGGCTATGGTGAAAAAAAAAAAAAA110.24433585073300756No Hit
ACGCTATGGTGTTCACGTACTCTGCGTTGATAC110.24433585073300756No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGAAA110.24433585073300756No Hit
GGCTATGGTGAAAAAAAAAAAAAAAAAAAAAAA100.2221235006663705No Hit
GCTATGGTGTAAAAAAAAAAAAAAAAAAAAAAA100.2221235006663705No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGTAA90.19991115059973344No Hit
GAGTGAATGGGGCTATGGTGAAAAAAAAAAAAA90.19991115059973344No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA80.1776988005330964No Hit
GGGCTATGGTGTAAAAAAAAAAAAAAAAAAAAA80.1776988005330964No Hit
GGCTATGGTGTTCACGTACTCTGCGTTGATACC80.1776988005330964No Hit
GTGAATGGGTGTTCACGTACTCAAAAAAAAAAA80.1776988005330964No Hit
CGCTATGGTGTTCACGTACTCTGCGTTGATACC80.1776988005330964No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTTCA80.1776988005330964No Hit
CGGCTATGGTGTTCACGTACTCTGCGTTGATAC80.1776988005330964No Hit
ATCAACGCAGAGTGAATGGGCTATGGTGAAAAA80.1776988005330964No Hit
ACGCAGAGTGAATGGGCTATGGTAAAAAAAAAA70.15548645046645934No Hit
ACGCAGAGTGAATGGGGCTATGGTGAAAAAAAA70.15548645046645934No Hit
TATCAACGCAGAGTGAATGGGGCTATGGTGAAA70.15548645046645934No Hit
GTGAATGGGGCTATGGTGTAAAAAAAAAAAAAA60.13327410039982232No Hit
TCGCTATGGTGTTCACGTACTCTGCGTTGATAC60.13327410039982232No Hit
ACGCAGAGTGAATGGGCTATGGTGTTCACGTAC60.13327410039982232No Hit
GGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13327410039982232No Hit
GTGAATGGGCTATGGAAAAAAAAAAAAAAAAAA60.13327410039982232No Hit
GTATCATCGCAGAGTGAATGGGCTATGGTGAAA60.13327410039982232No Hit
AGGCTATGGTGTTCACGTACTCTGCGTTGATAC60.13327410039982232No Hit
GTATCAACGCAGAGTGAATGGGGCTATGGTGAA60.13327410039982232No Hit
CCGCTATGGTGAAAAAAAAAAAAAAAAAAAAAA50.11106175033318524No Hit
GTGAATGGGGTACGTGAACACCAAAAAAAAAAA50.11106175033318524No Hit
TGGCTATGGTGTTCACGTACTCTGCGTTGATAC50.11106175033318524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11106175033318524No Hit
GAGCTATGGTGTAAAAAAAAAAAAAAAAAAAAA50.11106175033318524No Hit
GAGTGAATGGGCTATGGTGTAAAAAAAAAAAAA50.11106175033318524No Hit
GCTATGGTGTTCACGTACTCTTCGTTGATACCA50.11106175033318524No Hit
GAATGGGGCTATGGTGAAAAAAAAAAAAAAAAA50.11106175033318524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA256.6761207E-427.01
TGCGTTG700.001013040113.527
CTGCGTT800.002776624411.812526
TCTGCGT1255.8854447E-49.71999927
Produced by FastQC (version 0.11.2)