FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_15.3520000005fc69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_15.3520000005fc69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8283
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC1241.4970421344923337No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA270.3259688518652662No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT270.3259688518652662No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.3138959314258119No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA220.2656042496679947No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA200.2414584087890861No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA180.21731256791017745No Hit
GCTCAGCTATGCCAACGTACTCTGCGTTGATAC160.19316672703126886No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA160.19316672703126886No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT150.18109380659181454No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA140.16902088615236024No Hit
CCTCTCAGCTATGCCAACGTACTCTGCGTTGAT120.14487504527345166No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC110.13280212483399734No Hit
GCCTTCAGCTATGCCAACGTACTCTGCGTTGAT100.12072920439454304No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA100.12072920439454304No Hit
GCCCTCAGCTATGCCAACGTACTCTGCGTTGAT90.10865628395508872No Hit
CCCCCTCAGCTATGCCAACGTACTCTGCGTTGA90.10865628395508872No Hit
CCCATCAGCTATGCCAACGTACTCTGCGTTGAT90.10865628395508872No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA90.10865628395508872No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA90.10865628395508872No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT90.10865628395508872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT459.885445E-418.027
TATCAAC550.003785961314.7272721
ATCAACG600.006749425513.5000012
TCTGCGT1451.6354661E-611.17241427
CTGCGTT850.00496433911.11764724
CTCTGCG1751.3309291E-610.02857226
ACTCTGC1852.7195128E-69.48648625
TACTCTG1955.3337644E-69.024
GTACTCT2051.0082382E-58.56097523
ACGTACT2304.489817E-68.21739221
CGTACTC2304.489817E-68.21739222
AACGTAC2356.005819E-68.04255320
GCCAACG2356.005819E-68.04255317
CCAACGT2407.979301E-67.875000518
CAACGTA2407.979301E-67.875000519
TGCCAAC2304.3086162E-57.63043516
TATGCCA2355.6405916E-57.46808514
ATGCCAA2355.6405916E-57.46808515
GCTATGC2459.487474E-57.16326512
CTATGCC2459.487474E-57.16326513
Produced by FastQC (version 0.11.2)