FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_13.3520000005fc26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_13.3520000005fc26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6301
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATAGAGTCACGTACTCTGCGTTGATACCAC600.9522298047928901No Hit
CCACCATAGAGTCACGTACTCTGCGTTGATACC490.7776543405808601No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC450.7141723535946675No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC320.5078558958895413No Hit
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA240.38089192191715604No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC220.34915092842405965No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.26979844469131886No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA170.26979844469131886No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA140.22218695445167433No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA130.20631645770512616No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA120.19044596095857802No Hit
GTGAATGGGCACCATAGAGAAAAAAAAAAAAAA110.17457546421202982No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA100.15870496746548168No Hit
ACGCAGAGTGAATGGGCACCATAGAGTCAAAAA80.12696397397238532No Hit
GTGAATGGGCACCATAGAGTAAAAAAAAAAAAA70.11109347722583716No Hit
GAGTGAATGGGCACCATAGAGTCAAAAAAAAAA70.11109347722583716No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA70.11109347722583716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC350.00495714119.28571527
TTGATAC350.00495714119.28571526
GTGAATG656.2680314E-414.5384621
TGAATGG600.00651282313.5000012
TCTGCGT1705.8906153E-811.11764727
CTCTGCG2005.6069985E-79.45000126
ACTCTGC2151.5127262E-68.79069825
TACTCTG2252.8130726E-68.40000124
GTACTCT2355.0782455E-68.04255323
ACGTACT2355.0782455E-68.04255321
CGTACTC2355.0782455E-68.04255322
CACGTAC2406.75286E-67.875000520
GTCACGT2458.921386E-67.71428618
TCACGTA2458.921386E-67.71428619
AGTCACG2458.921386E-67.71428617
GAGTCAC2458.24539E-57.16326516
AGAGTCA2501.06076644E-47.0215
TAGAGTC2457.0585345E-46.61224514
CACCATA2702.753168E-46.59
ATAGAGT2508.898476E-46.4813
Produced by FastQC (version 0.11.2)