FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate7_10.3520000005fbba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate7_10.3520000005fbba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGAGACGTACGTACTCTGCGTTGATACCA612.824074074074074No Hit
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA301.3888888888888888No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA210.9722222222222222No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT180.8333333333333334No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA160.7407407407407408No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA130.6018518518518519No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA100.4629629629629629No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA100.4629629629629629No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA80.3703703703703704No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA80.3703703703703704No Hit
TATCAACGCAGAGTGAATGGGAGAGACGTACGT70.32407407407407407No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.32407407407407407No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA70.32407407407407407No Hit
ATGCAGAGACGTACGTACTCTTCGTTGATACCA60.2777777777777778No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA60.2777777777777778No Hit
ATGCAGAGACGTACGTAAAAAAAAAAAAAAAAA50.23148148148148145No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.23148148148148145No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA50.23148148148148145No Hit
TACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA50.23148148148148145No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA50.23148148148148145No Hit
ATGCAGAGACGTAAAAAAAAAAAAAAAAAAAAA50.23148148148148145No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.23148148148148145No Hit
GTGAATGGGATGCAGAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
ATCAACGCAGAGTGAATGGGGAGACGTACGTAC40.1851851851851852No Hit
GAGTGAATGGGATGCAGAGACAAAAAAAAAAAA40.1851851851851852No Hit
GTGAATGGGATGCAGAGACGAAAAAAAAAAAAA40.1851851851851852No Hit
GTGAATGGGAATGCAGAGACAAAAAAAAAAAAA40.1851851851851852No Hit
GACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
GACGTACGAAAAAAAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
GTGAATGGGATGCAGAGACAAAAAAAAAAAAAA40.1851851851851852No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA40.1851851851851852No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
GACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
GTATCAACGCAGAGTGAATGGGGAGACGTACGT40.1851851851851852No Hit
ATCAACGCAGAGTGAATGGGATGCAGAGAAAAA30.1388888888888889No Hit
CCTATGCAGAGACGTACGTACTCTGCGTTGATA30.1388888888888889No Hit
GTGAATGGGGAGAGACGTACGTACTCAAAAAAA30.1388888888888889No Hit
ACGCAGAGTGAATGGGAGAGACGTACGTACTCA30.1388888888888889No Hit
ATGCAGAGACAAAAAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
GATGCAGAGACGTACGTACTCTGCGTTGATACC30.1388888888888889No Hit
GCAGAGACGTACGTAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
GCAGAGACGTAAAAAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
GAGTGAATGGGAGACGTACGTACTCAAAAAAAA30.1388888888888889No Hit
GCAGAGACGTACGAAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGA30.1388888888888889No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT30.1388888888888889No Hit
CCCCCATGCAGAGACGTACGTACTCTGCGTTGA30.1388888888888889No Hit
GTGAATGGGATGCAGAGAAAAAAAAAAAAAAAA30.1388888888888889No Hit
GGAGACGTACGTACTCAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
ATGCAGAGACGAAAAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
GACGTACGTACTCTAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACAA30.1388888888888889No Hit
GAATGGGGAGACGTACGTACTCAAAAAAAAAAA30.1388888888888889No Hit
GTATCAACGCAGAGTGAATGGGATGCAGAGACG30.1388888888888889No Hit
ACGCAGAGTGAATGGGAGACGTACGTACTCAAA30.1388888888888889No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAATG600.004801433513.51
TGAATGG600.004801433513.52
GAATGGG650.00818230412.4615383
Produced by FastQC (version 0.11.2)