FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_95.3520000005fa5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_95.3520000005fa5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50240
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1980.39410828025477707No Hit
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA1390.276671974522293No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1230.2448248407643312No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA1060.21098726114649682No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1040.2070063694267516No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.1871019108280255No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA930.18511146496815287No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA910.18113057324840764No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT910.18113057324840764No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG900.17914012738853505No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA900.17914012738853505No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA870.1731687898089172No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT860.1711783439490446No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTA790.1572452229299363No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA750.14928343949044587No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA740.14729299363057327No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC680.13535031847133758No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAG620.12340764331210191No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA610.1214171974522293No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG580.11544585987261147No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.10947452229299363No Hit
GGCGAGAGACCGATAGCGAACAAGTACCGCGAG520.1035031847133758No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA510.10151273885350319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCC300.002239069722.4925145
ATCTACG559.396639E-619.6298312
TCTACGC559.396639E-619.6298313
TATCTAC559.396639E-619.6298311
ATCTCCA559.478263E-619.610266
CACTCCA559.478263E-619.610266
TGCAGCC509.969774E-518.931526
TACGCAG501.01243495E-418.8937135
CTACGCA501.01243495E-418.8937134
GAATGCA552.0688275E-417.21045523
GCAGCCC552.0688275E-417.21045527
ATGCAGC552.0688275E-417.21045525
GGGAATG653.9567E-516.60985821
AATGCAG604.0173173E-415.77624924
GGAATGC604.0173173E-415.77624922
TTGGGAA604.0790724E-415.744760519
TGGGAAT707.459408E-515.42343920
CGCTCCA707.523458E-515.4080616
TCTGCGT16250.014.72912327
TGTTTGG550.00417549214.72237416
Produced by FastQC (version 0.11.2)