FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_94.3520000005fa42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_94.3520000005fa42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89310
Sequences flagged as poor quality0
Sequence length33
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC3370.37733736423692754No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.3314298510805061No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA1960.21946030679655132No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA1840.20602396148247676No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC1660.1858694435113649No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG1600.1791512708543276No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA1570.17579218452580897No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA1480.16571492554025305No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA1340.1500391893404994No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA1260.14108162579778302No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT1210.13548314858358526No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT1210.13548314858358526No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT1160.12988467136938753No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA900.10077258985555929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTG200.00853220727.02355827
ATCTACG257.78235E-426.9932792
GTATCAT554.765752E-1026.9932791
CCCATCT257.78235E-426.9932794
TCTACGC257.78235E-426.9932793
TATCATC554.765752E-1026.9932792
CTACGCA257.78235E-426.9932794
CAGTAAG200.00857903926.99327716
TACGCAG307.09796E-526.9932775
GTAAGGT200.00857903926.99327718
AAGGTAC200.00857903926.99327720
ATCATCG405.9880404E-726.9932773
CGCGTCT200.00857903926.9932774
TAAGGTA200.00857903926.99327719
CCGCGTC200.00857903926.9932773
CATCGCA501.5341902E-724.2939515
TCATCGC451.672206E-623.9940244
TAACTTA352.0220144E-423.13709418
TATCTAC352.0220144E-423.1370941
TCCTTTC300.002251662822.49439811
Produced by FastQC (version 0.11.2)