FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_91.3520000005f9d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_91.3520000005f9d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55099
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGCTAGACGTACTCTGCGTTGATACCAC5731.0399462785168516No Hit
ACATCCAGCTAGACGTACTCTGCGTTGATACCA2110.3829470589302891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1880.3412040145919164No Hit
GTGAATGGGACATCCAGCAAAAAAAAAAAAAAA1170.21234505163433096No Hit
ATCCAGCTAGACGTACTCTGCGTTGATACCACT1070.19419590192199496No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT720.13067387792881904No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA700.12704404798635183No Hit
GTGAATGGGACATCCAGCTAGAAAAAAAAAAAA660.11978438810141745No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC660.11978438810141745No Hit
CCCACATCCAGCTAGACGTACTCTGCGTTGATA630.11433964318771665No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA570.10345015336031507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAT200.00852612227.009131
CTGCCAC200.00856423526.9844884
ACTACAT300.002244894222.4870746
ATACCAC1055.456968E-1219.30987227
GATACCA1152.3646862E-1117.63075326
TGATACC1152.3646862E-1117.63075325
TTGATAC1152.3646862E-1117.63075324
GCCACAT757.4044747E-616.1906936
TATCAAC2400.015.1926371
TCTGCGT9600.014.6433227
GTTGATA1404.7111826E-1014.48240423
CGACATC1252.530578E-814.0319347
GTATCAA1501.3460522E-913.5045661
ATCAACG2650.013.2376742
TCACATC1451.2430974E-813.0269967
TCAACGC2650.012.7285333
CTCTGCG12400.011.22775626
CAACGCA3050.011.05921654
ACTCTGC13200.010.44488525
CGTTGAT1956.4930646E-810.39762422
Produced by FastQC (version 0.11.2)