FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_89.3520000005f993.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_89.3520000005f993.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61403
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATCGGTTGAACGTACTCTGCGTTGATACCA3920.6384052896438285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.2247447193133886No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1080.175887171636565No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA970.15797273748839633No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA940.15308698272071397No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT920.1498298128755924No Hit
CCCTATCGGTTGAACGTACTCTGCGTTGATACC830.13517254857254532No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA830.13517254857254532No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA680.11074377473413351No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA670.10911518981157273No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA670.10911518981157273No Hit
GCCTATCGGTTGAACGTACTCTGCGTTGATACC650.10585801996645114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCT257.7677413E-426.9853175
TGAACCT257.7677413E-426.9853175
ACGCTGA300.002247520322.4877641
CGCTGAG300.002258214922.4694392
TCTGCGT16400.020.37110927
GATACCA701.750268E-719.3225127
GAGCCTA350.005491800619.2752296
ATGCCTA350.005491800619.2752296
CCATCCT350.005491800619.2752295
CGACCTA350.005491800619.2752296
AATCCTA602.005149E-517.9902116
GACCCTA552.1138918E-417.1724766
TATACCT500.002223180416.191195
CACCCTA500.002223180416.191196
TACCCTA500.002223180416.191196
CTGCCTA550.00419561214.7192656
TATCCTA657.5370044E-414.5305566
ACCCTAT2250.014.392177
CTTCCTA751.353282E-414.3921696
ATCCCTA751.353282E-414.3921696
Produced by FastQC (version 0.11.2)