FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_87.3520000005f950.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_87.3520000005f950.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31632
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC3070.9705361659079413No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT1190.37620131512392513No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA1180.37303995953464847No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA1170.36987860394537175No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA1030.32561962569549824No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT1020.3224582701062215No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC930.2940060698027314No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTA850.268715225088518No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG840.26555386949924126No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG840.26555386949924126No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.2623925139099646No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA810.2560698027314112No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC760.24026302478502784No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA750.23710166919575112No Hit
GGCGAGAGACCGATAGCGAACAAGTACCGCGAG700.22129489124936771No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT690.21813353566009105No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA690.21813353566009105No Hit
GTGAATGGGAGCTGACGAAAAAAAAAAAAAAAA680.21497218007081437No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAG620.19600404653515427No Hit
ATACGGGCGAGAGACCGATAGCGAACAAGTACC610.19284269094587758No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAAA590.18651997976732423No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTC570.18019726858877086No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT560.1770359129994942No Hit
GGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGC560.1770359129994942No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA550.17387455741021748No Hit
CTGTCGCACCACGAGGCGCTGTCTACGAGTCGG540.17071320182094082No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA530.16755184623166414No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA510.16122913505311076No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA490.15490642387455741No Hit
AGCTGACGAAGTACGTACTCTGCGTTGATACCA490.15490642387455741No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT490.15490642387455741No Hit
CTGGAGAAGCGTCCTCAGCGACGGACCGGGCCT490.15490642387455741No Hit
CAAGTACCGCGAGGTAAAAAAAAAAAAAAAAAA460.14542235710672738No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA450.14226100151745066No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGC450.14226100151745066No Hit
ATGCAGCCCCAATCGGGCGGTAAATTCCGTCCA430.13593829033889732No Hit
CTACGAGTCGGGTTGTTTGGGAATGCAGCCCCA410.12961557916034394No Hit
GTAAATTCCGTCCAAGGCTAAATACGGGCGAGA400.12645422357106728No Hit
GCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCG390.12329286798179058No Hit
GTCCTAAGATGAGCTCAACGAGAACAGAAATCT390.12329286798179058No Hit
CTGTCTACGAGTCGGGTTGTTTGGGAATGCAGC390.12329286798179058No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA390.12329286798179058No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAAA380.12013151239251392No Hit
CAGCTGACGAAGTACGTACTCTGCGTTGATACC380.12013151239251392No Hit
CTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCC370.11697015680323722No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAGT370.11697015680323722No Hit
CTGCAAAACCTAGGGCGCGAGCCCGGGCGGAGC370.11697015680323722No Hit
GTGAATGGGGCTGACGAAAAAAAAAAAAAAAAA360.11380880121396054No Hit
GTCGTGCCCGGACCCTGTCGCACCACGAGGCGC350.11064744562468386No Hit
GTACCGCGAGGTAAAAAAAAAAAAAAAAAAAAA350.11064744562468386No Hit
ATTCCGTCCAAGGCTAAATACGGGCGAGAGACC340.10748609003540718No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA340.10748609003540718No Hit
GAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGT340.10748609003540718No Hit
GAGTGAATGGGAGCTGACGAAAAAAAAAAAAAA340.10748609003540718No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA340.10748609003540718No Hit
GTTAAAAGATAACGCAGGTGTCCTAAGATGAGC330.1043247344461305No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAG330.1043247344461305No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCC330.1043247344461305No Hit
ACGCAGAGTGAATGGGGCTGACGAAAAAAAAAA330.1043247344461305No Hit
GTCGCACCACGAGGCGCTGTCTACGAGTCGGGT320.10116337885685382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGC300.002216524422.522
GCAAGAG300.002216524422.521
CGGGGCA300.002216524422.517
GGCAAGA300.002216524422.520
GCCAGGC300.002216524422.527
GGGGCAA300.002216524422.518
TCGGGGC300.002216524422.516
GTCGGGG404.880121E-420.2515
CCTATAC350.00541691319.2857152
CTATACC350.00541691319.2857153
GGGCAAG350.00541691319.28571519
AGCCAGG350.00541691319.28571526
AGAGCCA350.00541691319.28571524
CCCTTAG350.00541691319.2857151
TGCGTTG2250.016.227
TGTTTGG751.314395E-414.40000116
CAAGCTG852.3453726E-514.2941187
GTTTGGG904.0189796E-513.50000117
TTGGGAA904.0189796E-513.50000119
GCGTTGA904.0189796E-513.50000127
Produced by FastQC (version 0.11.2)