FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_85.3520000005f91c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_85.3520000005f91c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53899
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTACTAGAGACGTACTCTGCGTTGATACCAC3070.5695838512773892No Hit
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA2680.4972262936232584No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1560.2894302306165235No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA1370.25417911278502386No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA1300.24119185884710292No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.23562589287370825No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC990.18367687712202455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.17440026716636672No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA940.17440026716636672No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC780.14471511530826175No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA750.13914914933486705No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT640.11874060743241989No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA640.11874060743241989No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA610.11317464145902523No Hit
GTCGGTACTAGAGACGTACTCTGCGTTGATACC590.1094639974767621No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA580.10760867548563052No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA570.10575335349449896No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT570.10575335349449896No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA540.10018738752110429No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAG200.00853739427.000932
ACTCAGA200.00853739427.000933
TCAGAGT200.00853739427.000935
CTCAGAG200.00853739427.000934
ATGGGGA450.001091369318.000625
GGTATCA450.001091369318.000621
CTCTAGT857.03003E-817.471191
TCTGCGT4950.016.63693627
TAGTACC901.349581E-716.5005684
CTAGTAC901.349581E-716.5005683
ATACCAC500.002209824516.20055827
TCTAGTA1004.4670924E-714.8505112
TACCGAA657.4827456E-414.5389627
TCGGAAA904.1229403E-513.50046514
TATCAAC1901.8189894E-1213.5004651
GCGAGAG600.00742702313.50046513
GTACCGA1007.3432184E-613.5004646
GTATCAA1152.16035E-612.9134871
CGGAAAA1051.2146174E-512.85758615
CTTCGGT750.00220302112.6004347
Produced by FastQC (version 0.11.2)