FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_84.3520000005f8f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_84.3520000005f8f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80246
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTTCTGACGTACTCTGCGTTGATACCAC4730.5894374797497695No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.18567903696134386No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1360.16947885252847494No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC900.1121551229967849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCAG200.00853440927.01876322
TGATACC900.025.5177225
GATACCA950.024.1746826
ATACCAC1050.023.15893727
TCCACTG454.5537767E-520.988346
AGCACTG454.5537767E-520.988346
CTGCCAC350.00550659619.2750054
CTGCACT350.00550659619.2750055
CACCACT501.02388556E-418.8895075
TATCAAC1600.017.7310621
TCTGCGT16150.017.65002827
TGCACTG552.124313E-417.172286
CCACCAC552.124313E-417.172284
AGACTGT952.558918E-715.62297
TTGATAC1505.456968E-1215.31063224
ACACACT550.00421090714.7190965
CAACTGT1405.002221E-1014.4562557
GTTGATA1601.6370905E-1114.35371823
ATCAACG1800.014.250992
CTCTGCG19950.014.15268526
Produced by FastQC (version 0.11.2)