FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_8.3520000005ef76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_8.3520000005ef76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56419
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA9361.6590155798578494No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA4200.7444300678849324No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC4120.730250447544267No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA2550.451975398358709No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA2470.4377957780180436No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA2160.3828497491979652No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.359807866144384No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA1960.3474006983463018No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC1940.34385579326113547No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA1900.33676598309080275No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA1640.2906822169836403No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA1470.2605505237597263No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA1450.25700561867455995No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA1160.20560449493964797No Hit
GTATCATCGCAGAGTGAATGGGCACTGTACGTA1090.1931973271415658No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA1060.18787996951381628No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC930.16483808646023504No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA920.16306563391765186No Hit
TATCTACGCAGAGTGAATGGGCACTGTACGTAC910.16129318137506868No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT820.14534110849182014No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA810.14356865594923696No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA780.13825129832148744No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC770.13647884577890426No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC760.1347063932363211No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA670.11875432035307254No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA660.11698186781048939No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA640.11343696272532304No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA630.11166451018273986No Hit
GTACGTACCTAAAAAAAAAAAAAAAAAAAAAAA630.11166451018273986No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA580.10280224746982398No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC570.10102979492724082No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA570.10102979492724082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCCA200.00856152326.9875894
GTATCAT405.933289E-726.9875891
TATCATC405.933289E-726.9875892
TCGCAGA356.46071E-626.9875897
ATCATCG356.46071E-626.9875893
ATCGCAG356.46071E-626.9875896
CCGGCCA200.00856152326.9875894
TCATCGC401.8199276E-523.6141414
TCACATG300.002244205722.4896586
ACTACTC300.002244205722.4896583
TACTACT300.002244205722.4896582
CATCGCA454.5197943E-520.9903455
AACCCAT350.005483786619.276855
AGAATGG350.005483786619.2768514
GTACTAC1006.366463E-1118.8913121
GGTATCA651.8918636E-618.6837161
ATCTACG601.9990648E-517.9917262
TCTACGC601.9990648E-517.9917263
CTACGCA601.9990648E-517.9917264
TACGCAG552.1087499E-417.173925
Produced by FastQC (version 0.11.2)