FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_79.3520000005f851.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_79.3520000005f851.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48856
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1500.30702472572457834No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.2660880956279679No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA1100.22515146553135748No Hit
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA1070.2190109710168659No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA1050.21491730800720482No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA960.19649582446373015No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA930.1903553299492386No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA800.16374652038644177No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA800.16374652038644177No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA700.14327820533813657No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA660.13509087931881447No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT610.12485672179466185No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA570.11666939577533977No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT540.11052890126084822No Hit
CTTGCGATGTCTGACGTACTCTGCGTTGATACC530.1084820697560177No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA530.1084820697560177No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC530.1084820697560177No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA520.10643523825118716No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA510.10438840674635663No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA510.10438840674635663No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA490.1002947437366956No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA490.1002947437366956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCAGA200.00857396226.9723111
TGATACC653.8384875E-516.6667227
AGTTGCG500.002221529116.1833847
TCTGCGT6800.015.53313727
TTGATAC707.237424E-515.47623926
TATCAAC3150.014.5564841
TCAACGC3250.013.2786753
ATCAACG3150.013.2720882
CTCTGCG8300.012.725944526
GTATCAA2151.4551915E-1111.9179961
CAACGCA3900.011.4113614
ACTCTGC9950.010.61561125
AACGCAG4250.010.47160155
ACTTGCG1301.0836651E-410.3739657
GTTGATA1258.458303E-49.750030527
CGTTGAT1258.458303E-49.750030526
TACTCTG10900.09.69039824
GTACTCT11000.09.60230323
GCGTTGA1300.00121139149.3750325
GACGTAC12100.08.8140520
Produced by FastQC (version 0.11.2)