FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_76.3520000005f7f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_76.3520000005f7f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44175
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA3910.8851160158460668No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT2020.45727221279003966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.23542727787209963No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA950.21505376344086022No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA860.19468024900962083No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC770.17430673457838144No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC710.16072439162422186No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT710.16072439162422186No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA670.15166949632144877No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC670.15166949632144877No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA630.1426146010186757No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA620.14035087719298245No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA540.12224108658743632No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA520.11771363893604982No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA520.11771363893604982No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA510.11544991511035654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATA200.00857854426.963724
TAAAGAA200.00857854426.963722
AAAGAAC200.00857854426.963723
AGCCAGG351.9380316E-423.24352626
GAGCCAG351.9380316E-423.24352625
GCCAGGC351.9380316E-423.24352627
ATACCAC300.002174758422.59787226
TACCACT300.002174758422.59787227
ACCTATA404.929757E-420.2457451
CGGGGCA404.9677386E-420.2227917
CGTCGGG404.9677386E-420.2227914
GTCGGGG404.9677386E-420.2227915
TCGGGGC404.9677386E-420.2227916
CAAGAGC350.00531513219.36960622
AGAGCCA350.00531513219.36960624
GCAAGAG350.00545719619.2816621
GGGCAAG350.005493194319.259819
GGCAAGA350.005493194319.259820
GATACCA853.1341187E-919.14172627
TATACCC450.001097243317.9758114
Produced by FastQC (version 0.11.2)