FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_74.3520000005f7be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_74.3520000005f7be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45135
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACAGACAGTACGTACTCTGCGTTGATACCA7361.6306635648609724No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT4731.047967209482663No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA3170.7023374321480005No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2870.6358701672759499No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA2130.4719175805915587No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA2010.4453306746427385No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA1940.42982164617259333No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA1820.40323474022377315No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA1630.36113880580480784No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA1480.32790517336878255No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1440.3190428713858425No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA1310.29024038994128726No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC1210.2680846349839371No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA1170.259222333000997No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA1150.254791182009527No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA1150.254791182009527No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA1090.2414977290351169No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.23706657804364686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.22820427606070676No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA1020.22598870056497175No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA1000.22155754957350174No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA950.21047967209482663No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA950.21047967209482663No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.19718621912041653No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA880.19497064362468153No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA870.1927550681289465No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA860.19053949263321146No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGT820.1816771906502714No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA770.17059931317159632No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA750.16616816218012628No Hit
GTGAATGGGACACAGACAGTACAAAAAAAAAAA750.16616816218012628No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA710.1573058601971862No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA650.1440124072227761No Hit
GTGAATGGGGACACAGACAGAAAAAAAAAAAAA640.1417968317270411No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC620.13736568073557107No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGA600.13293452974410103No Hit
CAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA600.13293452974410103No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT580.128503378752631No Hit
GAATGGGGACACAGACAAAAAAAAAAAAAAAAA570.12628780325689598No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA570.12628780325689598No Hit
GGTATCAACGCAGAGTGAATGGGACACAGACAG550.12185665226542594No Hit
ACGCAGAGTGAATGGGACACAGACAGTAAAAAA540.11964107676969092No Hit
GACAGTACAAAAAAAAAAAAAAAAAAAAAAAAA540.11964107676969092No Hit
ATCAACGCAGAGTGAATGGGACACAGACAAAAA540.11964107676969092No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA520.1152099257782209No Hit
AGACAGTACGTACTCAAAAAAAAAAAAAAAAAA510.11299435028248588No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA510.11299435028248588No Hit
ACGCAGAGTGAATGGGACACAGACAGTACAAAA480.10634762379528083No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.10191647280381078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC356.4286196E-626.984462
TCGCAGA401.8109842E-523.6114047
ATCATCG401.8109842E-523.6114043
ATCGCAG401.8109842E-523.6114046
TCATCGC401.8109842E-523.6114044
CATCGCA401.8109842E-523.6114045
CACTGCT756.730261E-1021.65968927
CCACTGC756.730261E-1021.65968926
ACCACTG756.82121E-1021.63559525
ATACCAC801.5279511E-920.28337123
TACCACT801.5279511E-920.28337124
GTATCAT404.94433E-420.2383461
TATCAAC1800.017.2400721
GTATCAA807.193321E-716.8652881
ATCAACG2100.014.77720452
GATACCA1900.014.24979527
TGATACC2000.014.214169526
TCAACGC2250.013.7920583
GTTGATA2200.012.907598524
TTGATAC2200.012.907598525
Produced by FastQC (version 0.11.2)