FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_70.3520000005f738.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_70.3520000005f738.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37214
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC4121.107110227333799No Hit
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA3030.8142097060246144No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA2210.5938625248562369No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.5293706669532972No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA1890.5078733809856506No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA1630.43800720159079914No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC1590.42725855860697587No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC1590.42725855860697587No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA1510.4057612726393293No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA1510.4057612726393293No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA1300.349330896974257No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA1240.33320793249852204No Hit
GGTATCAACGCAGAGTGAATGGGTAGTTCTGAC980.2633417531036707No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA940.25259311011984736No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA900.2418444671360241No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA880.23647014564411242No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA870.2337829848981566No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA830.2230343419143333No Hit
GTATCAACGCAGAGTGAATGGGGTAGTTCTGAC780.2095985381845542No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA760.20422421669264254No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC680.18272693072499596No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA660.17735260923308432No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA610.1639168055033052No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA610.1639168055033052No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA600.16122964475734938No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCAAAAA590.15854248401139356No Hit
GTAGTTCTGACACGTACTCTGCGTTGATACCAC590.15854248401139356No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGAAAA590.15854248401139356No Hit
GTGAATGGGGTAGTTCTGACACGTACTCAAAAA580.15585532326543775No Hit
GTGAATGGGTAGTTCTGACAAAAAAAAAAAAAA580.15585532326543775No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA570.1531681625194819No Hit
GTTCTGACACGAAAAAAAAAAAAAAAAAAAAAA570.1531681625194819No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA560.15048100177352608No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACT550.1477938410275703No Hit
AGGTAGTTCTGACACGTACTCTGCGTTGATACC550.1477938410275703No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACGTAC530.14241951953565862No Hit
GACACGTACTCAAAAAAAAAAAAAAAAAAAAAA520.1397323587897028No Hit
ACGCAGAGTGAATGGGGTAGTTCAAAAAAAAAA510.13704519804374696No Hit
GTGAATGGGTAGTTCTGAAAAAAAAAAAAAAAA500.13435803729779117No Hit
GTGAATGGGGTAGTTCTGACACGTACAAAAAAA490.13167087655183535No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.13167087655183535No Hit
TGGTAGTTCTGACACGTACTCTGCGTTGATACC460.12360939431396785No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACG440.11823507282205621No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACA440.11823507282205621No Hit
TATCTACGCAGAGTGAATGGGTAGTTCTGACAC440.11823507282205621No Hit
GAGTGAATGGGGTAGTTCTGACACAAAAAAAAA430.11554791207610039No Hit
GAGTGAATGGGGTAGTTCTGACACGTACTCAAA430.11554791207610039No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACA410.11017359058418875No Hit
GGGTAGTTCTGACACGTACAAAAAAAAAAAAAA410.11017359058418875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT356.312501E-627.0256751
GAATGGT200.00852175426.98920416
AACTCAG356.379416E-626.9892042
TCAGAGT200.00852175426.9892045
TATCATC356.379416E-626.9892042
CAACTCA401.7784701E-523.6474651
ACTCAGA351.993121E-423.1336043
CTCAGAG351.993121E-423.1336044
TCGCAGA351.993121E-423.1336047
ATCATCG351.993121E-423.1336043
ATCGCAG351.993121E-423.1336046
TCATCGC351.993121E-423.1336044
CAACGGT351.993121E-423.1336045
CATCGCA351.993121E-423.1336045
TGATACC707.2996045E-921.23445927
GTATCAA1800.019.5185451
TTGATAC803.388959E-818.5801526
GTTGATA856.783739E-817.48720225
CCTGGTA703.7657264E-617.3502036
AACGGTA653.9140767E-516.6087426
Produced by FastQC (version 0.11.2)