FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_69.3520000005f72b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_69.3520000005f72b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91469
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC3580.3913894324853229No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC2260.24707824508849993No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT1730.18913511681553313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.1825755173884048No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC1650.1803889842460287No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAG1220.13337852168494244No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA1200.13119198854256633No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCG1090.11916605625949775No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT1080.1180727896883097No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA1030.11260645683236943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCACA200.00846380127.06875826
GTCCTAA257.781546E-426.9945141
GTAAGAC200.00860106426.9797153
CTACCCC200.00860106426.9797154
TACCCCA300.002258623722.4830975
CGGGGCA757.3305273E-1021.58377317
CGTCGGG757.3305273E-1021.58377314
TCGGGGC757.3305273E-1021.58377316
GCACCCA454.5696477E-520.9842225
AGTCCAG454.5696477E-520.9842226
TCTGCGT19450.020.66689327
AATCCAG405.00415E-420.2347876
TCCTAAG405.00415E-420.2347872
TTTCCAG405.00415E-420.2347876
CGTCCCA405.00415E-420.2347875
GGGGCAA751.7293132E-819.78512618
ACCTATA903.3105607E-1019.4960381
ACCCCAG903.3469405E-1019.485356
GGCAAGA853.4833647E-919.04450420
GTCGGGG853.4833647E-919.04450415
Produced by FastQC (version 0.11.2)