FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_66.3520000005f6bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_66.3520000005f6bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34212
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGGTATCCAAAAAAAAAAAAAAAAAA1990.5816672512568689No Hit
GTATCCAATGCACGTACTCTGCGTTGATACCAC1870.5465918391207764No Hit
GGTATCCAATGCACGTACTCTGCGTTGATACCA1610.47059511282590905No Hit
GTGAATGGGTATCCAAAAAAAAAAAAAAAAAAA1480.4325967496784754No Hit
CCGGTATCCAATGCACGTACTCTGCGTTGATAC1040.3039869051794692No Hit
GTGAATGGGTATCCAATGCACAAAAAAAAAAAA960.28060329708874077No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC850.24845083596398923No Hit
TATCAACGCAGAGTGAATGGGTATCCAATGCAC770.22506722787326086No Hit
GCGGTATCCAATGCACGTACTCTGCGTTGATAC770.22506722787326086No Hit
CTGGTATCCAATGCACGTACTCTGCGTTGATAC740.2162983748392377No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.2046065707938735No Hit
CCAATGCACGTACTCAAAAAAAAAAAAAAAAAA690.20168361978253244No Hit
GTGAATGGGTATCCAATGCACGTACTCAAAAAA650.18999181573716825No Hit
GGGTATCCAATGCACGTACTCTGCGTTGATACC600.175377060680463No Hit
CAGGTATCCAATGCACGTACTCTGCGTTGATAC590.17245410966912195No Hit
GTGAATGGGTATCCAATGCAAAAAAAAAAAAAA560.16368525663509878No Hit
ACGGTATCCAATGCACGTACTCTGCGTTGATAC550.16076230562375773No Hit
GTGGTATCCAATGCACGTACTCTGCGTTGATAC540.1578393546124167No Hit
ATGGTATCCAATGCACGTACTCTGCGTTGATAC530.15491640360107564No Hit
GTGAATGGGGTATCCAATGCACGTACTCAAAAA450.13153279551034724No Hit
GTGAATGGGTATCCAATGAAAAAAAAAAAAAAA450.13153279551034724No Hit
GGGGTATCCAATGCACGTACTCTGCGTTGATAC450.13153279551034724No Hit
TATCAACGCAGAGTGAATGGGGTATCCAAAAAA450.13153279551034724No Hit
ACGCAGAGTGAATGGGGTATCCAAAAAAAAAAA440.1286098444990062No Hit
TCGGTATCCAATGCACGTACTCTGCGTTGATAC440.1286098444990062No Hit
GAGTGAATGGGGTATCCAAAAAAAAAAAAAAAA420.1227639424763241No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT410.11984099146498303No Hit
TATCAACGCAGAGTGAATGGGTATCCAAAAAAA400.11691804045364199No Hit
CGGGTATCCAATGCACGTACTCTGCGTTGATAC390.11399508944230094No Hit
CGGTATCCAATGCACGTACTCTGCGTTGATACC370.10814918741961885No Hit
GTGAATGGGTATCCAATGCACGTAAAAAAAAAA360.1052262364082778No Hit
GTGAATGGGTATCCAATGCACGTACAAAAAAAA360.1052262364082778No Hit
GTATCAACGCAGAGTGAATGGGTATCCAATGCA350.10230328539693676No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC401.7714045E-523.65062327
TCTGCGT7350.015.996922527
TATCAAC1151.3265344E-714.1022351
GTATCAA903.997262E-513.5146421
TCGGTAT904.0529918E-513.4948837
TCAACGC1202.2710447E-713.4948833
ATCAACG1202.2710447E-713.4948832
GGGGTAT1552.0972948E-913.0595647
CTCTGCG9200.012.7801526
GCGGTAT853.824753E-412.7010667
CTGGTAT853.824753E-412.7010667
ATGGGTA1501.8946594E-812.5952235
CAGGTAT750.002181434512.5952237
CGGTATC4250.012.0660138
GTTGATA1708.123607E-911.92468426
ACTCTGC10000.011.89288525
TGATACC800.003500590811.82531225
ACGGTAT1153.0263467E-511.734687
TTGATAC1502.5692862E-711.71268927
TACTCTG10500.011.32655724
Produced by FastQC (version 0.11.2)