FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_61.3520000005f62c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_61.3520000005f62c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17167
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCATAGAGTCACGTACTCTGCGTTGATACC1460.8504689229335352No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC1210.7048406827051902No Hit
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA1180.6873652938777888No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC1010.5883380905225141No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC980.5708627016951127No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA840.4893108871672395No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA590.3436826469388944No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG520.30290673967495774No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA520.30290673967495774No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA510.297081610065824No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA510.297081610065824No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA480.27960622123842255No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC480.27960622123842255No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA420.24465544358361976No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA400.2330051843653521No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA370.21552979553795074No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA360.20970466592881692No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA360.20970466592881692No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTC360.20970466592881692No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT360.20970466592881692No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT350.2038795363196831No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT350.2038795363196831No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT340.1980544067105493No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA330.1922292771014155No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA310.1805790178831479No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.1805790178831479No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA270.15727849944661268No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC260.15145336983747887No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG250.14562824022834508No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT240.13980311061921127No Hit
ACGCAGAGTGAATGGGCACCATAGAGAAAAAAA240.13980311061921127No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA240.13980311061921127No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA240.13980311061921127No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA230.1339779810100775No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG220.12815285140094368No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG220.12815285140094368No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA210.12232772179180988No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA210.12232772179180988No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA210.12232772179180988No Hit
GTATCAACGCAGAGTGAATGGGCACCATAGAGT200.11650259218267606No Hit
GTATCAACGCAGAGTGAATGGGCACCATAGAGA200.11650259218267606No Hit
ACTCTATGGTGAAAAAAAAAAAAAAAAAAAAAA200.11650259218267606No Hit
GTGAATGGGCACCATAGAGAAAAAAAAAAAAAA200.11650259218267606No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA200.11650259218267606No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA200.11650259218267606No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG190.11067746257354227No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT190.11067746257354227No Hit
GTACGTGACTCTAAAAAAAAAAAAAAAAAAAAA190.11067746257354227No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA190.11067746257354227No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG180.10485233296440846No Hit
ATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGG180.10485233296440846No Hit
ACTCTATGGTGCAAAAAAAAAAAAAAAAAAAAA180.10485233296440846No Hit
GTGAATGGGCACCATAGAGTCAAAAAAAAAAAA180.10485233296440846No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA180.10485233296440846No Hit
CCATAGAGTCACAAAAAAAAAAAAAAAAAAAAA180.10485233296440846No Hit
CCATAGAGTCACGAAAAAAAAAAAAAAAAAAAA180.10485233296440846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACCA350.005317564619.2857157
TATCAAC852.910383E-919.0588251
ATCAACG952.2184395E-715.6315792
TCAACGC1056.903101E-714.1428573
CAACGCA1151.214521E-714.0869564
TCTGCGT4450.013.65168527
TGATACC700.001247783913.527
TTGATAC700.001247783913.526
TCACCAT956.333344E-512.7894748
GTTGATA800.003406303511.812525
TGCGTTG1604.0579835E-811.812527
CGTTGAT800.003406303511.812524
AACGCAG1307.596953E-611.4230775
CTCTGCG5350.011.3551426
CCCACCA1102.4882978E-411.0454557
ACTCTGC5500.011.04545425
GCGTTGA900.00819843210.523
TACTCTG5800.010.47413724
GTACTCT5850.010.38461523
CGTACTC6200.09.79838722
Produced by FastQC (version 0.11.2)