FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_6.3520000005ef33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_6.3520000005ef33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14466
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGATCAGACGTACTCTGCGTTGATACCAC1821.2581224941241531No Hit
GTGCTACGATCAGACGTACTCTGCGTTGATACC600.414765657403567No Hit
CGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA550.3802018526199364No Hit
GATCAGACGTACTCAAAAAAAAAAAAAAAAAAA520.35946356974975807No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT500.3456380478363058No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA460.3179870040094014No Hit
GAGTACGTCTGATCGTAGCAAAAAAAAAAAAAA460.3179870040094014No Hit
CTGCTACGATCAGACGTACTCTGCGTTGATACC340.23503387252868796No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA340.23503387252868796No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC330.22812111157196183No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA330.22812111157196183No Hit
GATCGTAGCAAAAAAAAAAAAAAAAAAAAAAAA290.20047006774505738No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA280.19355730678833125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.19355730678833125No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT240.16590626296142677No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA240.16590626296142677No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA240.16590626296142677No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA230.1589935020047007No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA230.1589935020047007No Hit
GTGAATGGGGTCTGATCGTAGCAAAAAAAAAAA210.14516798009124846No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA210.14516798009124846No Hit
GTCTGATCGTAGCAAAAAAAAAAAAAAAAAAAA200.13825521913452232No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA200.13825521913452232No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA200.13825521913452232No Hit
TATCAACGCAGAGTGAATGGGATCAGACGTACT190.13134245817779622No Hit
CCCCTGCTACGATCAGACGTACTCTGCGTTGAT180.1244296972210701No Hit
ATGCTACGATCAGACGTACTCTGCGTTGATACC170.11751693626434398No Hit
GTGAATGGGGAGTACGTCTGATCGTAGCAAAAA160.11060417530761786No Hit
GGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA160.11060417530761786No Hit
GTGCTACAAAAAAAAAAAAAAAAAAAAAAAAAA160.11060417530761786No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT150.10369141435089176No Hit
GTGAATGGGGGCGTGCAAAAAAAAAAAAAAAAA150.10369141435089176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGAGT300.002159004622.56
GGGAGTA350.00527814919.2857157
ATGGGAG601.8293129E-518.05
TGATACC657.026045E-414.53846227
TTGATAC700.001231240713.526
GTTGATA750.002071130812.59999925
TCTGCGT2900.012.10344927
AATGGGA1307.3666124E-611.4230784
TATCAAC1102.4370271E-411.0454551
GCGTTGA900.00809395510.50000123
CGTTGAT900.00809395510.50000124
ATCAACG1350.001606889.02
CTCTGCG3950.08.88607726
CTGCTAC3107.585186E-108.7096778
GTGAATG3353.43789E-108.4626871
TGAATGG3353.43789E-108.4626872
TCAACGC1450.0030777238.3793113
GAATGGG3652.0372681E-108.1369863
ACTCTGC4351.8189894E-128.06896525
CAGAGTG1700.00141135117.9411774
Produced by FastQC (version 0.11.2)