FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_58.3520000005f5b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_58.3520000005f5b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27010
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA2971.0995927434283599No Hit
ATGCAGAGACGTACGTACTCTGCGTTGATACCA2741.0144390966308774No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA1800.6664198445020363No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA1660.6145871899296557No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA1510.5590522028878193No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT1290.47760088855979266No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA1240.45908922621251386No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1060.3924472417623103No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA990.36653091447611996No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA980.3628285820066642No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA800.2961865975564606No Hit
GTATCAACGCAGAGTGAATGGGGAGACGTACGT780.28878193261754903No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT730.2702702702702703No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.2628656053313588No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA710.2628656053313588No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA540.1999259533506109No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGA500.18511662347278787No Hit
GTATCAACGCAGAGTGAATGGGATGCAGAGACG490.1814142910033321No Hit
TATCAACGCAGAGTGAATGGGAGAGACGTACGT460.17030729359496483No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.15920029618659756No Hit
GTGAATGGGATGCAGAGACAAAAAAAAAAAAAA400.1480932987782303No Hit
ATCAACGCAGAGTGAATGGGGAGACGTACGTAC400.1480932987782303No Hit
GAATGGGGAGACGTACGTACTCAAAAAAAAAAA400.1480932987782303No Hit
TATCAACGCAGAGTGAATGGGAGACGTACGTAC390.14439096630877452No Hit
GACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA380.1406886338393188No Hit
CAACGCAGAGTGAATGGGGAGACGTACGTACTC370.136986301369863No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACAA370.136986301369863No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA370.136986301369863No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG360.13328396890040725No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA360.13328396890040725No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.13328396890040725No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA350.1295816364309515No Hit
ATGCAGAGACGTAAAAAAAAAAAAAAAAAAAAA350.1295816364309515No Hit
GGAGACGTACGTACTCAAAAAAAAAAAAAAAAA340.12587930396149574No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT330.12217697149203999No Hit
GTGAATGGGATGCAGAGACGAAAAAAAAAAAAA330.12217697149203999No Hit
ATGCAGAGACAAAAAAAAAAAAAAAAAAAAAAA330.12217697149203999No Hit
GAGTGAATGGGATGCAGAGACAAAAAAAAAAAA330.12217697149203999No Hit
GACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.12217697149203999No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.11847463902258423No Hit
ATGCAGAGACGAAAAAAAAAAAAAAAAAAAAAA320.11847463902258423No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT320.11847463902258423No Hit
GTGAATGGGGATGCAGAGACAAAAAAAAAAAAA310.11477230655312846No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.11477230655312846No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA310.11477230655312846No Hit
GAGTGAATGGGATGCAGAGACGAAAAAAAAAAA300.11106997408367272No Hit
GGTATCAACGCAGAGTGAATGGGGAGACGTACG300.11106997408367272No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGAAAA300.11106997408367272No Hit
GTGAATGGGCATGCAGAGACAAAAAAAAAAAAA290.10736764161421695No Hit
GAGTGAATGGGAGACGTACGTACTCAAAAAAAA280.10366530914476121No Hit
GGAGAGACGTACGTACTCAAAAAAAAAAAAAAA280.10366530914476121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA257.6485303E-426.9888691
TGGGAAG300.002213459722.49072318
GTATCAA1100.019.6282671
GATACCA651.7848779E-618.71933227
ATGGGGT552.0568025E-417.1747345
TATCAAC2650.016.2951661
TGATACC756.990629E-616.22342126
TTGATAC756.990629E-616.22342125
ATCAACG2800.014.9402672
TCAACGC2850.014.6781583
CAACGCA3050.013.7156534
AACGCAG3500.011.9522135
GCGTTGA2101.0186341E-1011.58815727
ATGGGAA1051.6893882E-411.56665817
GTTGATA1102.5582133E-411.06142224
TGCGTTG3350.010.08919127
CGTTGAT1258.3243503E-49.73405223
ACGCAGA4700.09.4748156
CTGCGTT3600.09.38855326
ATGGGAG1752.065207E-59.2533265
Produced by FastQC (version 0.11.2)