FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_57.3520000005f597.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_57.3520000005f597.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44593
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC4951.1100396923283924No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC2340.5247460363734219No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA2010.45074339021819565No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC1880.42159083264189445No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC1700.381225752920862No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC920.20631040746305473No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC860.19285538088937726No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA700.1569753100262373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.1480052923104523No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA620.13903527459466733No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA620.13903527459466733No Hit
GTCTACGTACCGTAACGTACTCTGCGTTGATAC600.13455026573677484No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC580.13006525687888235No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA560.12558024802098985No Hit
CTCTACGTACCGTAACGTACTCTGCGTTGATAC560.12558024802098985No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT530.1188527347341511No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA520.11661023030520486No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA480.10764021258941987No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA480.10764021258941987No Hit
GCCTACGTACCGTAACGTACTCTGCGTTGATAC460.10315520373152737No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA460.10315520373152737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC1600.021.0937527
TTGATAC1950.017.30769326
GTTGATA2550.013.23529325
CGTTGAT2850.011.84210624
TATCAAC1204.7318514E-511.251
TCTGCGT8250.010.96363627
GCGTTGA3300.010.22727323
TCCTACG1351.5640684E-410.07
GTATCAA1300.00124178199.3461531
GCCTACG1905.5857363E-69.2368427
ATCAACG1350.00175222449.02
CTCTGCG11100.08.14864826
TCAACGC1500.00455102568.13
AATGGGG1700.0015640417.9411774
GTGAATG3703.750756E-97.6621621
ACTCTGC11850.07.518987725
TGCGTTG4556.366463E-117.417582522
TACTCTG12350.07.21457524
CCTACGT6750.07.28
GTAACGT12950.07.1930518
Produced by FastQC (version 0.11.2)