FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_54.3520000005f53a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_54.3520000005f53a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19267
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGATCAGACGTACTCTGCGTTGATACCAC2471.28198474074843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.4307883946644522No Hit
GTGCTACGATCAGACGTACTCTGCGTTGATACC780.40483728655213574No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT770.3996470649296725No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA750.3892666216847459No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG730.37888617843981937No Hit
GATCAGACGTACTCAAAAAAAAAAAAAAAAAAA700.3633155135724295No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA660.34255462708257645No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT630.32698396221518655No Hit
CTGCTACGATCAGACGTACTCTGCGTTGATACC620.3217937405927233No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT600.31141329734779677No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA590.30622307572533347No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA580.3010328541028702No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT560.29065241085794363No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA560.29065241085794363No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA550.2854621892354804No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA500.25951108112316396No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA470.24394041625577412No Hit
TATCAACGCAGAGTGAATGGGATCAGACGTACT470.24394041625577412No Hit
CGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA460.23875019463331085No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA440.22836975138838428No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA440.22836975138838428No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT430.223179529765921No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA420.21798930814345774No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA400.20760886489853114No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG390.20241864327606787No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA390.20241864327606787No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA390.20241864327606787No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA370.19203820003114133No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA370.19203820003114133No Hit
TGCTACGATCAGACGTACTCTGCGTTGATACCA330.17127731354128822No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG310.16089687029636165No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC310.16089687029636165No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA310.16089687029636165No Hit
GAGTACGTCTGATCGTAGCAAAAAAAAAAAAAA290.1505164270514351No Hit
ATGCTACGATCAGACGTACTCTGCGTTGATACC280.14532620542897182No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA280.14532620542897182No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT270.14013598380650855No Hit
GACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA270.14013598380650855No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG260.13494576218404525No Hit
CCCCTGCTACGATCAGACGTACTCTGCGTTGAT260.13494576218404525No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG250.12975554056158198No Hit
GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA240.1245653189391187No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG240.1245653189391187No Hit
GGATGGATGCCCGGGCATTGAGAAGGAAGGACG230.11937509731665542No Hit
GTGAATGGGGGCGTGCAAAAAAAAAAAAAAAAA230.11937509731665542No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA230.11937509731665542No Hit
GAGTGAATGGGATCAGACGTACTCAAAAAAAAA230.11937509731665542No Hit
GATACCGTCTGTGATCCATGGATCTCCGATCGG230.11937509731665542No Hit
CCCCGTTAGTAGCGGCGAGCGAGAGCGGATTTG220.11418487569419214No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA220.11418487569419214No Hit
GTATCAACGCAGAGTGAATGGGATCAGACGTAC210.10899465407172887No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC200.10380443244926557No Hit
GGAAATCAACCGAGACCCCGTTAGTAGCGGCGA200.10380443244926557No Hit
AAGGAAGGGAAATCAACCGAGACCCCGTTAGTA200.10380443244926557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA801.4006218E-920.2526
ATACCAC801.4006218E-920.2527
TTGATAC906.0572347E-918.024
GTTGATA1001.1386874E-917.5523
TGATACC951.1832526E-817.05263125
CGTTGAT1251.3005774E-915.1222
GCGTTGA1409.6899385E-812.53571421
TATCAAC1654.7002686E-912.2727271
GTATCAA1751.1270458E-811.5714281
ATCAACG1751.1270458E-811.5714282
ATGGGGA850.005399740311.1176475
TCAACGC1852.5653208E-810.9459463
AATGGGG2101.1914381E-910.9285724
CAACGCA1903.801506E-810.6578954
AACGCAG1955.5713826E-810.3846165
TGCGTTG1751.7459042E-610.02857120
GTGAATG4500.09.2999991
TGAATGG4550.09.1978032
CAGAGTG2502.1428241E-78.649
AGAGTGA2502.1428241E-78.6410
Produced by FastQC (version 0.11.2)