FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_53.3520000005f510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_53.3520000005f510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40692
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG1510.3710803106261673No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1470.3612503686228251No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC900.22117369507519907No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA800.1965988400668436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.18185392706183032No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT680.16710901405681708No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT610.14990661555096824No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA580.14253415904846162No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC510.1253317605426128No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA500.12287427504177725No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA440.10812936203676397No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT420.10321439103509289No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG410.10075690553425734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCCC451.5766709E-624.09362627
TGCAGCC451.5766709E-624.09362626
GAGTCGG658.0917744E-820.7474147
AGTCGGG658.0917744E-820.7474148
CGAGTCG658.0917744E-820.7474146
GTCGGGT658.198367E-820.7218639
TTGGGAA608.6057844E-720.25370419
TGTTTGG608.8130946E-720.2038216
CGGGTTG608.8130946E-720.2038211
TCGGGTT608.8130946E-720.2038210
TCTACGA751.6412741E-819.8036212
TGGGAAT701.7453385E-719.28924220
ACGAGTC701.7682942E-719.2654575
TACGAGT701.7682942E-719.2654574
GTCTACG651.8757328E-618.6726721
GAATGCA601.9034691E-518.07021923
ATGCAGC601.9034691E-518.07021925
AATGCAG601.9034691E-518.07021924
GTTTGGG601.9655345E-518.00329417
CTACGAG753.652858E-717.9810923
Produced by FastQC (version 0.11.2)