FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_52.3520000005f4f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_52.3520000005f4f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15185
Sequences flagged as poor quality0
Sequence length33
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG2851.876852156733619No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA2681.7648995719459992No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA2631.7319723411261114No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT2211.4553836022390516No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA2161.4224563714191638No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA2091.3763582482713204No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT1921.264405663483701No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT1711.1261112940401712No Hit
GTATCGACGTCTACGTACTCTGCGTTGATACCA1601.0536713862364175No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT1400.9219624629568653No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA1290.8495225551531116No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA1240.8165953243332237No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC1140.7507408626934475No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA970.6387882779058282No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG960.6322028317418505No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG950.6256173855778729No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG910.5992756009219624No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA910.5992756009219624No Hit
GGATGGATGCCCGGGCATTGAGAAGGAAGGACG750.49390846229832075No Hit
TAGTAGCGGCGAGCGAGAGCGGATTTGGGATTT740.4873230161343431No Hit
ACCGAGACCCCGTTAGTAGCGGCGAGCGAGAGC740.4873230161343431No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG720.4741521238063879No Hit
CCCCGTTAGTAGCGGCGAGCGAGAGCGGATTTG710.46756667764241033No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA710.46756667764241033No Hit
GAGATACCGTCTGTGATCCATGGATCTCCGATC660.43463944682252226No Hit
CTTTGAAAACTTAGCGAACTGAAACATCTAAGT650.4280540006585446No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT640.42146855449456705No Hit
CCCGTTAGTAGCGGCGAGCGAGAGCGGATTTGG640.42146855449456705No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA600.39512676983865663No Hit
CCGTATCCAAGCTCCGTGGCTAGTCTGCGCTCT600.39512676983865663No Hit
GATACCGTCTGTGATCCATGGATCTCCGATCGG600.39512676983865663No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG590.388541323674679No Hit
GAAACATCTAAGTAGCTAAAGGAAGGGAAATCA570.37537043134672377No Hit
CCATGGATCTCCGATCGGGAAACCGTATCCAAG550.36219953901876856No Hit
GGGGAGATACCGTCTGTGATCCATGGATCTCCG530.3490286466908133No Hit
ATCCATGGATCTCCGATCGGGAAACCGTATCCA520.3424432005268357No Hit
GGCCATGGGGAGATACCGTCTGTGATCCATGGA510.3358577543628581No Hit
AACTGAAACATCTAAGTAGCTAAAGGAAGGGAA500.3292723081988805No Hit
GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA470.30951596970694767No Hit
AATCAACCGAGACCCCGTTAGTAGCGGCGAGCG460.30293052354297006No Hit
CTGAAACATCTAAGTAGCTAAAGGAAGGGAAAT430.2831741850510372No Hit
GGAAATCAACCGAGACCCCGTTAGTAGCGGCGA420.2765887388870596No Hit
GAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCC420.2765887388870596No Hit
TCTAAGTAGCTAAAGGAAGGGAAATCAACCGAG410.270003292723082No Hit
GAAGGGAAATCAACCGAGACCCCGTTAGTAGCG400.2634178465591044No Hit
CATTGGATGGATGCCCGGGCATTGAGAAGGAAG400.2634178465591044No Hit
GTTAGTAGCGGCGAGCGAGAGCGGATTTGGGAT380.2502469542311492No Hit
CTGTGATCCATGGATCTCCGATCGGGAAACCGT380.2502469542311492No Hit
CGGGAAACCGTATCCAAGCTCCGTGGCTAGTCT370.24366150806717154No Hit
CGCTTTCAGAGGCGAAAGGCCATGGGGAGATAC360.23707606190319394No Hit
ATGGATCTCCGATCGGGAAACCGTATCCAAGCT360.23707606190319394No Hit
ATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGG340.22390516957523873No Hit
GATCCATGGATCTCCGATCGGGAAACCGTATCC340.22390516957523873No Hit
AAGGAAGGGAAATCAACCGAGACCCCGTTAGTA340.22390516957523873No Hit
GTCTGTGATCCATGGATCTCCGATCGGGAAACC340.22390516957523873No Hit
AACCGAGACCCCGTTAGTAGCGGCGAGCGAGAG330.21731972341126113No Hit
GTGATCCATGGATCTCCGATCGGGAAACCGTAT310.20414883108330592No Hit
CCGTGGCTAGTCTGCGCTCTTTGGACTTTGAAA300.19756338491932832No Hit
GTATCAACGCAGAGTGAATGGGGAATGCATTGG300.19756338491932832No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA290.19097793875535068No Hit
CCGTTAGTAGCGGCGAGCGAGAGCGGATTTGGG290.19097793875535068No Hit
TTTGAAAACTTAGCGAACTGAAACATCTAAGTA280.18439249259137308No Hit
TATCAACGCAGAGTGAATGGGGAATGCATTGGA270.17780704642739548No Hit
GGGAAACCGTATCCAAGCTCCGTGGCTAGTCTG270.17780704642739548No Hit
AGCGAGAGCGGATTTGGGATTTTAAAAAAAAAA260.17122160026341784No Hit
GGAAGGACGCTTTCAGAGGCGAAAGGCCATGGG260.17122160026341784No Hit
ACGCAGAGTGAATGGGGAATGCATTGGATGGAT250.16463615409944024No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA240.15805070793546264No Hit
TCCGTGGCTAGTCTGCGCTCTTTGGACTTTGAA230.15146526177148503No Hit
GAAACATCTAAGTAGCTAAAAAAAAAAAAAAAA230.15146526177148503No Hit
ATGGATGCCCGGGCATTGAGAAGGAAGGACGCT230.15146526177148503No Hit
GATCTCCGATCGGGAAACCGTATCCAAGCTCCG230.15146526177148503No Hit
CCGATCGGGAAACCGTATCCAAGCTCCGTGGCT230.15146526177148503No Hit
TACCGTCTGTGATCCATGGATCTCCGATCGGGA230.15146526177148503No Hit
TCGGGAAACCGTATCCAAGCTCCGTGGCTAGTC220.1448798156075074No Hit
GCGCTCTTTGGACTTTGAAAACTTAGCGAACTG220.1448798156075074No Hit
GTAGCGGCGAGCGAGAGCGGATTTGGGATTTTA220.1448798156075074No Hit
TCTTTGGACTTTGAAAACTTAGCGAACTGAAAC220.1448798156075074No Hit
GGCTAGTCTGCGCTCTTTGGACTTTGAAAACTT210.1382943694435298No Hit
GATTTGGGATTTTAAGAAAAAAAAAAAAAAAAA200.1317089232795522No Hit
AACTGAAACATCTAAGTAGCTAAAAAAAAAAAA200.1317089232795522No Hit
CTTTCAGAGGCGAAAGGCCATGGGGAGATACCG200.1317089232795522No Hit
TTTCAGAGGCGAAAGGCCATGGGGAGATACCGT200.1317089232795522No Hit
TGGGGAGATACCGTCTGTGATCCATGGATCTCC200.1317089232795522No Hit
CGGCGAGCGAGAGCGGATTTGGGATTTTAAAAA200.1317089232795522No Hit
GGAATGCATTGGATGGATGCCCGGGCATTGAGA200.1317089232795522No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA190.1251234771155746No Hit
AAGTAGCTAAAGGAAGGGAAATCAACCGAGACC190.1251234771155746No Hit
CCGAGACCCCGTTAGTAGCGGCGAGCGAGAGCG180.11853803095159697No Hit
GAAGGAAGGACGCTTTCAGAGGCGAAAGGCCAT180.11853803095159697No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA180.11853803095159697No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA180.11853803095159697No Hit
ACTTAGCGAACTGAAACATCTAAGTAGCTAAAG180.11853803095159697No Hit
CTTAGCGAACTGAAACATCTAAGTAGCTAAAGG180.11853803095159697No Hit
GTATCCAAGCTCCGTGGCTAGTCTGCGCTCTTT180.11853803095159697No Hit
CCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG180.11853803095159697No Hit
AAAGGAAGGGAAATCAACCGAGACCCCGTTAGT170.11195258478761937No Hit
GGTATCGACGTCTACGTACTCTGCGTTGATACC160.10536713862364176No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA160.10536713862364176No Hit
GGAAGGGAAATCAACCGAGACCCCGTTAGTAGC160.10536713862364176No Hit
CGTCTGTGATCCATGGATCTCCGATCGGGAAAC160.10536713862364176No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA160.10536713862364176No Hit
AGAGCGGATTTGGGATTTTAAAAAAAAAAAAAA160.10536713862364176No Hit
TAGCTAAAGGAAGGGAAATCAACCGAGACCCCG160.10536713862364176No Hit
CATCTAAGTAGCTAAAGGAAGTGAAATCAACCG160.10536713862364176No Hit
TCTCCGATCGGGAAACCGTATCCAAGCTCCGTG160.10536713862364176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAT756.6712782E-616.2019881
GGAGATA801.2313934E-515.1893632
AAGTAGC801.2313934E-515.1893636
ATCTAAG801.2313934E-515.1893632
TAGCTAA801.2313934E-515.1893639
GTAGCTA801.27115945E-515.1392328
TAAGTAG852.185694E-514.2958715
CATCTAA852.185694E-514.2958711
TCTAAGT852.185694E-514.2958713
TCTGCGT1456.2755134E-1013.96722927
TGCGTTG600.00710174113.50165627
AGTAGCT903.7474034E-513.5016567
AGCTAAA903.7474034E-513.50165610
CTAAGTA956.22945E-512.7910424
AGATACC853.5925402E-412.707444
AAAGGAA1001.00722056E-412.15148914
CTAAAGG1001.00722056E-412.15148912
TAAAGGA1001.00722056E-412.15148913
GAGATAC905.8187137E-412.00147153
GCTAAAG1051.588295E-411.57284711
Produced by FastQC (version 0.11.2)