FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_51.3520000005f4e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_51.3520000005f4e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43215
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTTCCTCACGTACTCTGCGTTGATACCA6081.40691889390258No Hit
CTCTGTTCCTCACGTACTCTGCGTTGATACCAC2080.48131435844035636No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC970.22445909984958926No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT550.12727062362605576No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA540.12495661228740021No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT510.11801457827143352No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA500.11570056693277796No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC500.11570056693277796No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA490.1133865555941224No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA490.1133865555941224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACT300.002247059722.4700685
GATACCA851.4006218E-1020.71560727
TGATACC851.4006218E-1020.71560726
ACGACTC350.005490883719.2600576
ATCAACG951.31367415E-817.0497022
TATCAAC1002.4741894E-816.1972161
TCAACGC1002.5100235E-816.1784483
CAACGCA1054.575486E-815.4080464
GCGTTGA3100.014.8556127
TTGATAC1201.3762474E-814.67355525
GTTGATA1252.3563189E-814.08661324
CGCACTC600.007462880613.482046
AACGCAG1304.1945896E-813.4820395
CGACTCT1051.2184259E-512.8400387
GCACTCT1359.800297E-711.98403557
CGTTGAT1502.5551526E-711.73884423
GACTCTG2303.6379788E-1211.7235148
TCTGCGT10200.011.68572727
ACACTCT1051.7503822E-411.5560347
CCACTCT2059.877112E-1011.1802287
Produced by FastQC (version 0.11.2)