FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_50.3520000005f4ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_50.3520000005f4ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22089
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.5794739463081172No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA990.4481868803476844No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT920.4164968989089592No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA830.3757526370591697No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA780.3531169360315089No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA740.33500837520938026No Hit
CCCTAGCTCACTACCACGTACTCTGCGTTGATA740.33500837520938026No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA680.30784553397618725No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA650.29426411335959074No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA530.23993843089320477No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA530.23993843089320477No Hit
TAGCTCACTACCACGTACTCTGCGTTGATACCA370.16750418760469013No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA350.15844990719362578No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT320.1448684865770293No Hit
CCCCTAGCTCACTACCACGTACTCTGCGTTGAT320.1448684865770293No Hit
GTGGTAGTGAGCTAAAAAAAAAAAAAAAAAAAA310.14034134637149712No Hit
ACCTAGCTCACTACCACGTACTCTGCGTTGATA310.14034134637149712No Hit
GTAGTGAGCTAAAAAAAAAAAAAAAAAAAAAAA310.14034134637149712No Hit
CCTTAGCTCACTACCACGTACTCTGCGTTGATA290.1312870659604328No Hit
CCCCCTAGCTCACTACCACGTACTCTGCGTTGA290.1312870659604328No Hit
ACGTGGTAGTGAGCTAAAAAAAAAAAAAAAAAA270.12223278554936846No Hit
GTACGTGGTAGTGAGCTAAAAAAAAAAAAAAAA270.12223278554936846No Hit
GCCTAGCTCACTACCACGTACTCTGCGTTGATA250.11317850513830414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA1050.020.60779227
ACTAGCT802.2346876E-413.4931977
CGTTGAT1705.2568794E-1012.72834326
TATCAAC1401.0020085E-712.5293981
TCAACGC1502.4622932E-711.6941043
ATCAACG1502.4622932E-711.6941042
CAACGCA1502.4622932E-711.6941044
TCTGCGT6350.010.43573727
TGCGTTG3550.010.28575527
GCGTTGA2254.267713E-89.6169725
CTGCGTT4000.09.46670426
AACGCAG1752.0131973E-59.2524785
GTGAATG3601.9826984E-108.2458431
AATGGGG2009.672939E-58.0959194
CTCTGCG8200.08.08133326
TGAATGG3354.0654413E-98.055642
ACGCAGA2552.8407558E-67.9371751
ATAGCTC2101.7066418E-47.71039878
ACTCTGC9000.07.362992325
CGCAGAG2409.7294804E-57.30881557
Produced by FastQC (version 0.11.2)