HTC23BCXX_l01n02_kb_plate6_5.3520000005ef19.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HTC23BCXX_l01n02_kb_plate6_5.3520000005ef19.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53839
Sequences flagged as poor quality	0
Sequence length	33
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC	269	0.49963780902319876	No Hit
ACGTAGCTCACGTACTCTGCGTTGATACCACTG	185	0.343617080554988	No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA	120	0.22288675495458682	No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA	118	0.21917197570534372	No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG	113	0.2098850275822359	No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA	109	0.2024554690837497	No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT	99	0.18388157283753412	No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA	93	0.1727372350898048	No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA	92	0.17087984546518323	No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT	86	0.15973550771745387	No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT	83	0.1541633388435892	No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCC	81	0.1504485595943461	No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA	74	0.13744683222199522	No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG	72	0.1337320529727521	No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA	72	0.1337320529727521	No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTC	69	0.1281598840988874	No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAGCTC	68	0.12630249447426586	No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC	67	0.12444510484964431	No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA	66	0.12258771522502276	No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT	63	0.11701554635115809	No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA	63	0.11701554635115809	No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTA	62	0.11515815672653651	No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA	61	0.11330076710191496	No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAA	59	0.10958598785267186	No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC	58	0.1077285982280503	No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA	58	0.1077285982280503	No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA	58	0.1077285982280503	No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA	57	0.10587120860342875	No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAG	57	0.10587120860342875	No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGC	57	0.10587120860342875	No Hit
CTGGAGAAGCGTCCTCAGCGACGGACCGGGCCT	56	0.10401381897880718	No Hit
ACGTAGCTCAAAAAAAAAAAAAAAAAAAAAAAA	55	0.10215642935418563	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACTCA	40	5.826223E-7	27.039145	1
GTATCAT	55	4.5656634E-10	27.039145	1
TATCTAC	60	4.1836756E-11	27.039143	1
TACCACT	25	7.665241E-4	27.039143	26
ATCTACG	60	4.3655746E-11	26.988838	2
TACGCAG	55	4.656613E-10	26.988838	5
TCTACGC	65	1.1823431E-10	24.912773	3
TATCATC	60	1.2805685E-9	24.739767	2
CTACGCA	60	1.2805685E-9	24.739767	4
TCATCGC	55	1.389526E-8	24.535307	4
ATCGCAG	55	1.4033503E-8	24.512505	6
CATCGCA	50	1.512999E-7	24.289953	5
ACCACTG	30	0.0022180486	22.53262	27
ATCATCG	60	3.49919E-8	22.490698	3
TCGCAGA	60	3.5339326E-8	22.469795	7
AACTCAG	50	4.130725E-6	21.591068	2
GGTTGTT	80	3.5308403E-8	18.554825	13
GGGTTGT	80	3.5308403E-8	18.554825	12
CGGGTTG	80	3.5308403E-8	18.554825	11
TCGGGTT	80	3.5308403E-8	18.554825	10

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content