FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate6_49.3520000005f4a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate6_49.3520000005f4a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43935
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA10492.387618072152043No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA8041.8299761010583817No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA6451.4680778422669853No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA4481.0196881757141232No Hit
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA4471.0174120860361897No Hit
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA4310.9809946511892568No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA3450.7852509388869922No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA3000.6828269033799932No Hit
GACACACGTACTCAAAAAAAAAAAAAAAAAAAA2940.6691703653123933No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGT2840.6464094685330602No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.6213724820757938No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA2420.5508137020598612No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA2380.5417093433481279No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA2350.5348810743143281No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA2260.5143962672129282No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA2190.498463639467395No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA1930.4392853078411289No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGT1910.43473312848526235No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA1910.43473312848526235No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA1880.4279048594514624No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.42107659041766243No Hit
CACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.402867872994196No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA1750.39831569363832936No Hit
GAGTGAATGGGCAGACACACGTACTCAAAAAAA1660.37783088653692953No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC1440.32775691362239673No Hit
TATCTACGCAGAGTGAATGGGCAGACACACGTA1430.32548082394446337No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACACG1410.32092864458859677No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA1410.32092864458859677No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT1410.32092864458859677No Hit
GTATCATCGCAGAGTGAATGGGCAGACACAAAA1380.31410037555479686No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA1370.31182428587686356No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA1300.2958916581313304No Hit
CACACGTACTCTAAAAAAAAAAAAAAAAAAAAA1300.2958916581313304No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA1270.28906338909753043No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.28906338909753043No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA1260.28678729941959713No Hit
CACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA1250.28451120974166383No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA1190.27085467167406396No Hit
TATCTACGCAGAGTGAATGGGCAGACACAAAAA1150.2617503129623307No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACAAA1030.23443723682713102No Hit
CAGACACACGTACTCAAAAAAAAAAAAAAAAAA1020.2321611471491977No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA1020.2321611471491977No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA990.22533287811539776No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA960.2185046090815978No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.21622851940366453No Hit
ACGCAGAGTGAATGGGCAGACACACGTAAAAAA860.1957437123022647No Hit
GTGAATGGGCAGACACACGTAAAAAAAAAAAAA850.1934676226243314No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGA850.1934676226243314No Hit
CAACTCAGAGTGAATGGGCAGACACAAAAAAAA840.1911915329463981No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA810.18436326391259816No Hit
CAACGCAGAGTGAATGGGCAGACACACGTACTC790.17981108455673153No Hit
CAACTCAGAGTGAATGGGCAGACACACGTACTC780.17753499487879823No Hit
GTATCAACGCAGAGTGAATGGGCAGACAAAAAA770.1752589052008649No Hit
AACGCAGAGTGAATGGGCAGACACACGAAAAAA760.1729828155229316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.1729828155229316No Hit
CACACGAAAAAAAAAAAAAAAAAAAAAAAAAAA700.15932627745533173No Hit
AACGCAGAGTGAATGGGCAGACACACAAAAAAA680.1547740980994651No Hit
GAGTGAATGGGCAGACACACGAAAAAAAAAAAA670.1524980084215318No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.1502219187435985No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACAA660.1502219187435985No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA650.1479458290656652No Hit
GACACACAAAAAAAAAAAAAAAAAAAAAAAAAA620.14111756003186526No Hit
GTATCATCGCAGAGTGAATGGGCAGACAAAAAA620.14111756003186526No Hit
CAACTCAGAGTGAATGGGCAGACAAAAAAAAAA610.13884147035393193No Hit
CGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.13884147035393193No Hit
CACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.1342892909980653No Hit
GAGTGAATGGGCAGACACACGTAAAAAAAAAAA580.132013201320132No Hit
GAGTGAATGGGCAGACACACAAAAAAAAAAAAA560.1274610219642654No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.1274610219642654No Hit
AACGCAGAGTGAATGGGCAGACACACGTAAAAA550.1251849322863321No Hit
CACACGTAAAAAAAAAAAAAAAAAAAAAAAAAA540.12290884260839877No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCTG530.12063275293046546No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCTA530.12063275293046546No Hit
TATCTACGCAGAGTGAATGGGCAGACAAAAAAA510.11608057357459885No Hit
GGCAGACACACGTACTCAAAAAAAAAAAAAAAA510.11608057357459885No Hit
AACGTAGAGTGAATGGGCAGACACAAAAAAAAA500.11380448389666552No Hit
GCAGACACACGTACTCAAAAAAAAAAAAAAAAA500.11380448389666552No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGAAA490.11152839421873222No Hit
ACACACGTACTCAAAAAAAAAAAAAAAAAAAAA480.1092523045407989No Hit
ATCAACGCAGAGTGAATGGGCAGACAAAAAAAA480.1092523045407989No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1092523045407989No Hit
AACGTAGAGTGAATGGGCAGACACACGTACTCA470.1069762148628656No Hit
ACGCTGAGTGAATGGGCAGACACAAAAAAAAAA460.1047001251849323No Hit
CCCATGAGCAGACACACGTACTCTGCGTTGATA450.10242403550699897No Hit
CACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA450.10242403550699897No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCT450.10242403550699897No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGA440.10014794582906567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT1000.026.9863011
TATCATC1000.026.9863012
GGTATCA700.026.9863011
GAGTAAA200.00854296226.98631
AGTAAAT200.00854296226.98632
ATCTACG451.6436243E-623.9878222
TCTACGC451.6436243E-623.9878223
TACGCAG451.6436243E-623.9878225
TATCTAC451.6436243E-623.9878221
CTACGCA451.6436243E-623.9878224
GCTATGA300.002236864522.4885856
ATCATCG1250.021.5890433
TCATCGC1250.021.5890434
TCGCAGA1200.021.3641557
ATCGCAG1200.021.3641556
CATCGCA1200.021.3641555
TGCGTTG3300.018.03203827
GTATCAA2250.017.9908681
ACGCTGA450.001090933517.9908661
CTGAGTG552.0959394E-417.1731014
Produced by FastQC (version 0.11.2)